ProtParam
User-provided sequence:
10 20 30 40 50 60
MDPTSRPAIV IDNGTGYTKM GFAGNVEPCF ILPTVVAVNE SFLNQSKSSS KATWQTQHNA
70 80 90 100 110 120
GVAADLDFYI GDEALAKSRS SSTHNLHYPI EHGQVEDWDA MERYWQQCIF NYLRCDPEDH
130 140 150 160 170 180
YFLLTESPLT PPESREYTGE ILFETFNVPG LYIAVNSVLA LAAGYTTSKC EMTGVVVDVG
190 200 210 220 230 240
DGATHVVPVA EGYVIGSCIK SIPIAGKDVT LFIQQLMRER GENIPPEDSF DVARKVKEMY
250 260 270 280 290 300
CYTCSDIVKE FNKHDKEPAK YIKQWKGVKP KTGAPYTCDV GYERFLGPEV FFNPEIYSND
310 320 330 340 350 360
FTTTLPAVID KCIQSAPIDT RRALYKNIVL SGGSTMFKDF GRRLQRDLKK IVDARVLANN
370 380 390 400 410 420
ARTGGEITSQ PVEVNVVSHP VQRFAVWFGG SVLSSTPEFF ASCRTKEEYE EYGASICRTN
PVFKGMY
References and
documentation are available.
Number of amino acids: 427
Molecular weight: 47659.97
Theoretical pI: 5.43
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 2133
Hydrogen H 3274
Nitrogen N 560
Oxygen O 643
Sulfur S 19
Formula: C
2133H
3274N
560O
643S
19
Total number of atoms: 6629
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57925
Abs 0.1% (=1 g/l) 1.215, assuming all pairs of Cys residues form cystines
Ext. coefficient 57300
Abs 0.1% (=1 g/l) 1.202, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.85
This classifies the protein as unstable.
Aliphatic index: 74.85
Grand average of hydropathicity (GRAVY): -0.275