ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDQASSSSTM EEDKKSSTDP VNVDDNEERE AIEIALFQVP ECYVYLIPPR KTAASYRADE 

        70         80         90        100        110        120 
WDVNKWAWEG ALKVVSKGEE CIIKLVDKTT GELYAQAFLR EGELHPVEAV IDSSRYFVLR 

       130        140        150        160        170        180 
VEEKIDGRVR HAFIGLGFRE RTEAYDFQAA LHDHMKYLNK KKTAEEMEQH YQNTSSVDYS 

       190        200        210        220        230        240 
LKEGETIVLQ LKNRSDKDSK SKTVEKSLGN LSLEDKGKSI ETTIPSIILP PPPPGPLSPV 

       250        260        270        280        290        300 
TTAQKSPSSL PPSLSLQRSS EQQDLDTKRE EEEEEKKEDL KAKDGGVEDA PDDDFGDFQA 


AG 

References and documentation are available.

Number of amino acids: 302

Molecular weight: 33917.68

Theoretical pI: 4.75

Amino acid composition: 
Ala (A)  21	  7.0%
Arg (R)  13	  4.3%
Asn (N)   7	  2.3%
Asp (D)  25	  8.3%
Cys (C)   2	  0.7%
Gln (Q)  12	  4.0%
Glu (E)  36	 11.9%
Gly (G)  15	  5.0%
His (H)   5	  1.7%
Ile (I)  13	  4.3%
Leu (L)  24	  7.9%
Lys (K)  27	  8.9%
Met (M)   4	  1.3%
Phe (F)   8	  2.6%
Pro (P)  17	  5.6%
Ser (S)  28	  9.3%
Thr (T)  15	  5.0%
Trp (W)   3	  1.0%
Tyr (Y)   9	  3.0%
Val (V)  18	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1484
Hydrogen    H	      2344
Nitrogen    N	       400
Oxygen      O	       496
Sulfur      S	         6

Formula: C1484H2344N400O496S6
Total number of atoms: 4730

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30035
Abs 0.1% (=1 g/l)   0.886, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.882, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.99
This classifies the protein as unstable.



Aliphatic index: 72.02

Grand average of hydropathicity (GRAVY): -0.802