ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDQDFDSLLL GFNDSDSVQK DPTVPNGLDG SVVDPTIADP TAITARKRRP QVKLTAEKLL 

        70         80         90        100        110        120 
SDKGLPYVLK NAHKRIRISS KKNSYDNLSN IIQFYQLWAH ELFPKAKFKD FMKICQTVGK 

       130        140        150        160        170        180 
TDPVLREYRV SLFRDEMGMS FDVGTRETGQ DLERQSPMVE EHVTSAEERP IVADSFAQDK 

       190        200        210        220        230        240 
RNVNNVDYDN DEDDDIYHLS YRNRRGRVLD ERGNNETVLN NVVPPKEDLD ALLKTFRVQG 

       250        260        270        280        290        300 
PVGLEENEKK LLLGWLDAHR KMEKGSMTEE DVQLIQSLEE WEMNDIEGQH THYDLLPGGD 

       310 
EFGVDQDELD AMKEMGF 

References and documentation are available.

Number of amino acids: 317

Molecular weight: 36354.63

Theoretical pI: 4.77

Amino acid composition: 
Ala (A)  13	  4.1%
Arg (R)  19	  6.0%
Asn (N)  17	  5.4%
Asp (D)  35	 11.0%
Cys (C)   1	  0.3%
Gln (Q)  14	  4.4%
Glu (E)  27	  8.5%
Gly (G)  19	  6.0%
His (H)   7	  2.2%
Ile (I)  11	  3.5%
Leu (L)  31	  9.8%
Lys (K)  22	  6.9%
Met (M)  10	  3.2%
Phe (F)  12	  3.8%
Pro (P)  14	  4.4%
Ser (S)  17	  5.4%
Thr (T)  14	  4.4%
Trp (W)   3	  0.9%
Tyr (Y)   8	  2.5%
Val (V)  23	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1588
Hydrogen    H	      2498
Nitrogen    N	       444
Oxygen      O	       512
Sulfur      S	        11

Formula: C1588H2498N444O512S11
Total number of atoms: 5053

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28420
Abs 0.1% (=1 g/l)   0.782, assuming all pairs of Cys residues form cystines


Ext. coefficient    28420
Abs 0.1% (=1 g/l)   0.782, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.17
This classifies the protein as unstable.



Aliphatic index: 76.81

Grand average of hydropathicity (GRAVY): -0.768