ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDQEALLVGR TLLKRGIPTI PFILASQALE KLAYFGLVPN MILFLTVEYG MGTAEAANIL 

        70         80         90        100        110        120 
FLWSAATNFF PLVGAFIADS YTGRFPLIGF GSSISLTGMV LLWLTTIIRP ECDKLTNVCQ 

       130        140        150        160        170        180 
PTTLLKSVLL YSFFALTAIG AGGVRSSCLA FAADQLQPNQ TSRVTTSSLE TLFNWYYFSV 

       190        200        210        220        230        240 
MVACFLSQSL LVFVQTTYGW QIGFGVSVAA MALSVALFFA ASPYYVRFQK PTRNSRNPWK 

       250        260        270        280        290        300 
LCRVQQVEDL KSLINVIPIW STGIILSLVT ACQVSFIVLQ AKTMDRHTFI QGFEIPPGSY 

       310        320        330        340        350        360 
GIFLVISFLL FLGLYDLVIV PLLSWALREP FRLGVMVRMW AGYVISVLCI SALAATEYAR 

       370        380        390        400        410        420 
RKTARDESGT KLSAMWLLPY MILGGIAEAL NTIAQNEFFY SELPKTMSSV ATTLSSLNMA 

       430        440        450        460        470        480 
AASLISSWII TIVDVTTYGS WITENIDEGH LDYYYWLLVG LSLLNVLYFV WCKKSYGKCS 


I 

References and documentation are available.

Number of amino acids: 481

Molecular weight: 53385.77

Theoretical pI: 8.47

Amino acid composition: 
Ala (A)  41	  8.5%
Arg (R)  17	  3.5%
Asn (N)  14	  2.9%
Asp (D)  11	  2.3%
Cys (C)   9	  1.9%
Gln (Q)  16	  3.3%
Glu (E)  16	  3.3%
Gly (G)  30	  6.2%
His (H)   2	  0.4%
Ile (I)  35	  7.3%
Leu (L)  67	 13.9%
Lys (K)  14	  2.9%
Met (M)  13	  2.7%
Phe (F)  30	  6.2%
Pro (P)  18	  3.7%
Ser (S)  43	  8.9%
Thr (T)  35	  7.3%
Trp (W)  13	  2.7%
Tyr (Y)  21	  4.4%
Val (V)  36	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      2486
Hydrogen    H	      3845
Nitrogen    N	       593
Oxygen      O	       665
Sulfur      S	        22

Formula: C2486H3845N593O665S22
Total number of atoms: 7611

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   103290
Abs 0.1% (=1 g/l)   1.935, assuming all pairs of Cys residues form cystines


Ext. coefficient   102790
Abs 0.1% (=1 g/l)   1.925, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.31
This classifies the protein as stable.



Aliphatic index: 112.93

Grand average of hydropathicity (GRAVY): 0.608