ProtParam
User-provided sequence:
10 20 30 40 50 60
MDQEIEIPSF FLCPISLDIM KDPVIVSTGI TYDRESIEKW LFSGKKNSCP VTKQVITETD
70 80 90 100 110 120
LTPNHTLRRL IQSWCTLNAS YGIERIPTPK PPICKSEIEK LIKESSSSHL NQVKCLKRLR
130 140 150 160 170 180
QIVSENTTNK RCLEAAEVPE FLANIVSNSV DTYNSPSSSL SSSNLNDMCQ SNMLENRFDS
190 200 210 220 230 240
SRSLMDEALS VLYHLDTSET ALKSLLNNKK GTNLVKTLTK IMQRGIYESR AYAALLLKKL
250 260 270 280 290 300
LEVADPMQII LLERELFGEV IQILHDQISH KATRSAMQIL VITCPWGRNR HKAVEGGTIS
310 320 330 340 350 360
MIIELLMDDT FSSERRNSEM AMVVLDMLCQ CAEGRAEFLN HGAAIAVVSK KILRVSQITS
370 380 390 400 410 420
ERAVRVLLSV GRFCATPSLL QEMLQLGVVA KLCLVLQVSC GNKTKEKAKE LLKLHARVWR
430
ESPCVPRNLY DSYPA
References and
documentation are available.
Number of amino acids: 435
Molecular weight: 48876.87
Theoretical pI: 8.56
Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2143
Hydrogen H 3528
Nitrogen N 596
Oxygen O 646
Sulfur S 28
Formula: C
2143H
3528N
596O
646S
28
Total number of atoms: 6941
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34795
Abs 0.1% (=1 g/l) 0.712, assuming all pairs of Cys residues form cystines
Ext. coefficient 33920
Abs 0.1% (=1 g/l) 0.694, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.74
This classifies the protein as unstable.
Aliphatic index: 101.29
Grand average of hydropathicity (GRAVY): -0.115