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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDQKASYFIN EKLFTEVKPV LFTDLIHHLK IGPSMAKKLM FDYYKQTTNA KYNCVVICCY

70 80 90 100 110 120
KDQTIKIIHD LSNIPQQDSI IDCFIYAFNP MDSFIPYYDI IDQKDCLTIK NSYELKVSES

130 140 150 160 170 180
SKIIERTKTL EEKSKPLVRP TARSKTTPEE TTGRKSKSKD MGLRSTALLA KMKKDRDDKE

190 200 210 220 230 240
TSRQNELRKR KEENLQKINK QNPEREAQMK ELNNLFVEDD LDTEEVNGGS KPNSPKETDS

250 260 270 280 290 300
NDKDKNNDDL EDLLETTAED SLMDVPKIQQ TKPSETEHSK EPKSEEEPSS FIDEDGYIVT

310 320 330 340 350
KRPATSTPPR KPSPVVKRAL SSSKKQETPS SNKRLKKQGT LESFFKRKAK


References and documentation are available.

Number of amino acids: 350 Molecular weight: 40309.65 Theoretical pI: 8.51
Amino acid composition: 
Ala (A) 12 3.4% Arg (R) 15 4.3% Asn (N) 18 5.1% Asp (D) 28 8.0% Cys (C) 5 1.4% Gln (Q) 14 4.0% Glu (E) 32 9.1% Gly (G) 7 2.0% His (H) 4 1.1% Ile (I) 21 6.0% Leu (L) 25 7.1% Lys (K) 49 14.0% Met (M) 8 2.3% Phe (F) 11 3.1% Pro (P) 21 6.0% Ser (S) 32 9.1% Thr (T) 26 7.4% Trp (W) 0 0.0% Tyr (Y) 10 2.9% Val (V) 12 3.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 60 Total number of positively charged residues (Arg + Lys): 64 Atomic composition: Carbon C 1757 Hydrogen H 2852 Nitrogen N 484 Oxygen O 571 Sulfur S 13 Formula: C1757H2852N484O571S13 Total number of atoms: 5677 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 15150 Abs 0.1% (=1 g/l) 0.376, assuming all pairs of Cys residues form cystines Ext. coefficient 14900 Abs 0.1% (=1 g/l) 0.370, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 53.49 This classifies the protein as unstable. Aliphatic index: 64.63 Grand average of hydropathicity (GRAVY): -1.047