ProtParam
User-provided sequence:
10 20 30 40 50 60
MDQKASYFIN EKLFTEVKPV LFTDLIHHLK IGPSMAKKLM FDYYKQTTNA KYNCVVICCY
70 80 90 100 110 120
KDQTIKIIHD LSNIPQQDSI IDCFIYAFNP MDSFIPYYDI IDQKDCLTIK NSYELKVSES
130 140 150 160 170 180
SKIIERTKTL EEKSKPLVRP TARSKTTPEE TTGRKSKSKD MGLRSTALLA KMKKDRDDKE
190 200 210 220 230 240
TSRQNELRKR KEENLQKINK QNPEREAQMK ELNNLFVEDD LDTEEVNGGS KPNSPKETDS
250 260 270 280 290 300
NDKDKNNDDL EDLLETTAED SLMDVPKIQQ TKPSETEHSK EPKSEEEPSS FIDEDGYIVT
310 320 330 340 350
KRPATSTPPR KPSPVVKRAL SSSKKQETPS SNKRLKKQGT LESFFKRKAK
References and
documentation are available.
Number of amino acids: 350
Molecular weight: 40309.65
Theoretical pI: 8.51
Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 1757
Hydrogen H 2852
Nitrogen N 484
Oxygen O 571
Sulfur S 13
Formula: C
1757H
2852N
484O
571S
13
Total number of atoms: 5677
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15150
Abs 0.1% (=1 g/l) 0.376, assuming all pairs of Cys residues form cystines
Ext. coefficient 14900
Abs 0.1% (=1 g/l) 0.370, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.49
This classifies the protein as unstable.
Aliphatic index: 64.63
Grand average of hydropathicity (GRAVY): -1.047