ProtParam
User-provided sequence:
10 20 30 40 50 60
MDQLSDSYAL YNQPVVIDNG SGIIKAGFSG EERPKALEYC LVGNTKYDKV MLEGLQGDTF
70 80 90 100 110 120
IGNNAQKLRG LLKLRYPIKH GVVEDWDSME LIWSYVLNEV LQLQNIGEHP LLITEAPMNP
130 140 150 160 170 180
LKNREQMAQV LFETFDVSAL YVSNPAVLSL YASGRTTGCV VDCGEGYCST VPIYDGFALP
190 200 210 220 230 240
ASMMRMDIGG ADITEQLQFQ LRKSAGVSLF SSSEREIVRT MKEKVCYLAK NIKKEEEKYL
250 260 270 280 290 300
QGTQDLISTF KLPDGRCIEV GNDRYRAPEI LFSPQIIGLG YDGLSDMCMQ SIWKVDLDLR
310 320 330 340 350 360
KPLLSSIILS GGTTTLKGFG DRMLWDLEAL TKGTSKIKII APSERKYTTW IGGSILTGLS
370 380
TFQRLWTKKS DWLEDSTRVY SNLM
References and
documentation are available.
Number of amino acids: 384
Molecular weight: 42995.50
Theoretical pI: 5.40
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1919
Hydrogen H 3046
Nitrogen N 500
Oxygen O 577
Sulfur S 20
Formula: C
1919H
3046N
500O
577S
20
Total number of atoms: 6062
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 62715
Abs 0.1% (=1 g/l) 1.459, assuming all pairs of Cys residues form cystines
Ext. coefficient 62340
Abs 0.1% (=1 g/l) 1.450, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.04
This classifies the protein as stable.
Aliphatic index: 93.41
Grand average of hydropathicity (GRAVY): -0.171