ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDQNLYRKCL VILSMMAMIG TSMATYAGTP WRTASATFYG DDTGSATMGG ACGYGNMYDS 

        70         80         90        100        110        120 
GYGVATTALS TALFNEGYAC GQCFQLKCVS SPNCYYGSPA TVVTATNICP PNYGQASNNG 

       130        140        150        160        170        180 
GWCNPPRVHF DLTKPAFMKI ANWKAGIIPV SYRRVACKKI GGIRFKFEGN GYWLLVYVMN 

       190        200        210        220        230        240 
VGGPGDIKTM AVKGSRTGWI NMSHNWGASY QAFSSLYGQS LSFRLTSYTT RQTIYAYNAA 

       250 
PASWSAGKTY QSKANFN 

References and documentation are available.

Number of amino acids: 257

Molecular weight: 27869.72

Theoretical pI: 9.36

Amino acid composition: 
Ala (A)  27	 10.5%
Arg (R)   9	  3.5%
Asn (N)  17	  6.6%
Asp (D)   6	  2.3%
Cys (C)   9	  3.5%
Gln (Q)   8	  3.1%
Glu (E)   2	  0.8%
Gly (G)  29	 11.3%
His (H)   2	  0.8%
Ile (I)  11	  4.3%
Leu (L)  12	  4.7%
Lys (K)  12	  4.7%
Met (M)  11	  4.3%
Phe (F)  10	  3.9%
Pro (P)  11	  4.3%
Ser (S)  22	  8.6%
Thr (T)  21	  8.2%
Trp (W)   7	  2.7%
Tyr (Y)  19	  7.4%
Val (V)  12	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	      1242
Hydrogen    H	      1870
Nitrogen    N	       332
Oxygen      O	       361
Sulfur      S	        20

Formula: C1242H1870N332O361S20
Total number of atoms: 3825

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67310
Abs 0.1% (=1 g/l)   2.415, assuming all pairs of Cys residues form cystines


Ext. coefficient    66810
Abs 0.1% (=1 g/l)   2.397, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.30
This classifies the protein as stable.



Aliphatic index: 58.95

Grand average of hydropathicity (GRAVY): -0.141