ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDRDISYQQN YTSTGATATS SRQPSTDNNA DTNFLKVMSE FKYNFNSPLP TTTQFPTPYS 

        70         80         90        100        110        120 
SNQYQQTQDH FANTDAHNSS SNESSLVENS ILPHHQQIQQ QQQQQQQQQQ QQQALGSLVP 

       130        140        150        160        170        180 
PAVTRTDTSE TLDDINVQPS SVLQFGNSLP SEFLVASPEQ FKEFLLDSPS TNFNFFHKTP 

       190        200        210        220        230        240 
AKTPLRFVTD SNGAQQSTTE NPGQQQNVFS NVDLNNLLKS NGKTPSSSCT GAFSRTPLSK 

       250        260        270        280        290        300 
IDMNLMFNQP LPTSPSKRFS SLSLTPYGRK ILNDVGTPYA KALISSNSAL VDFQKARKDI 

       310        320        330        340        350        360 
TTNATSIGLE NANNILQRTP LRSNNKKLFI KTPQDTINST STLTKDNENK QDIYGSSPTT 

       370        380        390        400        410        420 
IQLNSSITKS ISKLDNSRIP LLASRSDNIL DSNVDDQLFD LGLTRLPLSP TPNCNSLHST 

       430        440        450        460        470        480 
TTGTSALQIP ELPKMGSFRS DTGINPISSS NTVSFKSKSG NNNSKGRIKK NGKKPSKFQI 

       490        500        510        520        530        540 
IVANIDQFNQ DTSSSSLSSS LNASSSAGNS NSNVTKKRAS KLKRSQSLLS DSGSKSQARK 

       550 
SCNSKSNGNL FNSQ 

References and documentation are available.

Number of amino acids: 554

Molecular weight: 60522.65

Theoretical pI: 9.50

Amino acid composition: 
Ala (A)  24	  4.3%
Arg (R)  18	  3.2%
Asn (N)  57	 10.3%
Asp (D)  29	  5.2%
Cys (C)   3	  0.5%
Gln (Q)  47	  8.5%
Glu (E)  11	  2.0%
Gly (G)  21	  3.8%
His (H)   6	  1.1%
Ile (I)  25	  4.5%
Leu (L)  48	  8.7%
Lys (K)  36	  6.5%
Met (M)   5	  0.9%
Phe (F)  25	  4.5%
Pro (P)  33	  6.0%
Ser (S)  92	 16.6%
Thr (T)  50	  9.0%
Trp (W)   0	  0.0%
Tyr (Y)   8	  1.4%
Val (V)  16	  2.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2598
Hydrogen    H	      4160
Nitrogen    N	       760
Oxygen      O	       889
Sulfur      S	         8

Formula: C2598H4160N760O889S8
Total number of atoms: 8415

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12045
Abs 0.1% (=1 g/l)   0.199, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.197, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.29
This classifies the protein as unstable.



Aliphatic index: 64.10

Grand average of hydropathicity (GRAVY): -0.781