ProtParam
User-provided sequence:
10 20 30 40 50 60
MDRDTKLAFR LRGSHSRRTD DIDDDVIVFK TPNAVYREEN SPIQSPVQPI LSSPKLANSF
70 80 90 100 110 120
EFPITTNNVN AQDRHEHGYQ PLDAEDYPMI DSENKSLISE SPQNVRNDED LTTRYNFDDI
130 140 150 160 170 180
PIRQLSSSIT SVTTIDVLSS LFINLFENDL IPQALKDFNK SDDDQFRKLL YKLDLRLFQT
190 200 210 220 230 240
ISDQMTRDLK DILDINVSNN ELCYQLKQVL ARKEDLNQQI ISVRNEIQEL KAGKDWHDLQ
250 260 270 280 290 300
NEQAKLNDKV KLNKRLNDLT STLLGKYEGD RKIMSQDSED DSIRDDSNIL DIAHFVDLMD
310 320
PYNGLLKKIN KINENLSNEL QPSL
References and
documentation are available.
Number of amino acids: 324
Molecular weight: 37461.81
Theoretical pI: 4.73
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1633
Hydrogen H 2612
Nitrogen N 458
Oxygen O 538
Sulfur S 6
Formula: C
1633H
2612N
458O
538S
6
Total number of atoms: 5247
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.465, assuming all pairs of Cys residues form cystines
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.465, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.23
This classifies the protein as unstable.
Aliphatic index: 91.76
Grand average of hydropathicity (GRAVY): -0.744