ProtParam
User-provided sequence:
10 20 30 40 50 60
MDRESALRLE TPRRELISRA RSFSLATSKN RCKKMDNEKA LRFELECRRT GKCREIVADV
70 80 90 100 110 120
QHSRMPRHME QEACSLAAKS IMTYHLEHDI ARHLKMAFDR EYGPDWHCIC GKHFGSFVTF
130 140 150 160 170 180
EPDSFIYFRI GTIAFMLFKT SLQRLPIMEK HLENVKLTPT TILGRSRLMT PKSEDDEEST
SASDVS
References and
documentation are available.
Number of amino acids: 186
Molecular weight: 21646.89
Theoretical pI: 8.76
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 940
Hydrogen H 1506
Nitrogen N 278
Oxygen O 279
Sulfur S 15
Formula: C
940H
1506N
278O
279S
15
Total number of atoms: 3018
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10345
Abs 0.1% (=1 g/l) 0.478, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.461, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.26
This classifies the protein as unstable.
Aliphatic index: 68.76
Grand average of hydropathicity (GRAVY): -0.566