ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDRLGSFSND PSDKPPCRGC SSYLMEPYIK CAECGPPPFF LCLQCFTRGF EYKKHQSDHT 

        70         80         90        100        110        120 
YEIMTSDFPV LDPSWTAQEE MALLEAVMDC GFGNWQDVAN QMCTKTKEEC EKHYMKHFIN 

       130        140        150        160        170        180 
NPLFASTLLN LKQAEEAKTA DTAIPFHSTD DPPRPTFDSL LSRDMAGYMP ARADFIEEFD 

       190        200        210        220        230        240 
NYAERDLRDI DFVEDDSDIL HALKMAVVDI YHSRLKERQR RKKIIRDHGL INLRKFQLME 

       250        260        270        280        290        300 
RRYPKEVQDL YETMRRFARI VGPVEHDKFI ESHALEFELR REIKRLQEYR TAGITNFCSA 

       310        320        330        340        350        360 
RTYNHLKKTR EEERLKRTML SEVLQYIQDS SACQQWLRRQ AGIDSGLSPS IPMASNSGRR 

       370        380        390        400        410        420 
SAPPLNLTGL PGTEKLNEKE KELCQMVRLV PGAYLEYKSA LLNECNKQGG LRLAQARALI 

       430        440 
KIDVNKTRKI YDFLIREGYI TKG 

References and documentation are available.

Number of amino acids: 443

Molecular weight: 51406.75

Theoretical pI: 7.14

Amino acid composition: 
Ala (A)  29	  6.5%
Arg (R)  36	  8.1%
Asn (N)  16	  3.6%
Asp (D)  29	  6.5%
Cys (C)  13	  2.9%
Gln (Q)  18	  4.1%
Glu (E)  37	  8.4%
Gly (G)  20	  4.5%
His (H)  11	  2.5%
Ile (I)  23	  5.2%
Leu (L)  43	  9.7%
Lys (K)  30	  6.8%
Met (M)  15	  3.4%
Phe (F)  20	  4.5%
Pro (P)  23	  5.2%
Ser (S)  26	  5.9%
Thr (T)  21	  4.7%
Trp (W)   3	  0.7%
Tyr (Y)  17	  3.8%
Val (V)  13	  2.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2262
Hydrogen    H	      3564
Nitrogen    N	       640
Oxygen      O	       674
Sulfur      S	        28

Formula: C2262H3564N640O674S28
Total number of atoms: 7168

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42580
Abs 0.1% (=1 g/l)   0.828, assuming all pairs of Cys residues form cystines


Ext. coefficient    41830
Abs 0.1% (=1 g/l)   0.814, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.33
This classifies the protein as unstable.



Aliphatic index: 73.16

Grand average of hydropathicity (GRAVY): -0.629