ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDRPIDDIVK NLLKFVVRGF YGGSFVLVLD AILFHSVLAE DDLKQLLSIN KTELGPLIAR 

        70         80         90        100        110        120 
LRSDRLISIH KQREYPPNSK SVERVYYYVK YPHAIDAIKW KVHQVVQRLK DDLDKNSEPN 

       130        140        150        160        170        180 
GYMCPICLTK YTQLEAVQLL NFDRTEFLCS LCDEPLVEDD SGKKNKEKQD KLNRLMDQIQ 

       190        200        210        220        230        240 
PIIDSLKKID DSRIEENTFE IALARLIPPQ NQSHAAYTYN PKKGSTMFRP GDSAPLPNLM 

       250        260        270        280        290        300 
GTALGNDSSR RAGANSQATL HINITTASDE VAQRELQERQ AEEKRKQNAV PEWHKQSTIG 

       310        320        330        340        350        360 
KTALGRLDNE EEFDPVVTAS AMDSINPDNE PAQETSYQNN RTLTEQEMEE RENEKTLNDY 

       370        380        390        400        410        420 
YAALAKKQAK LNKEEEEEEE EEEDEEEEEE EEMEDVMDDN DETARENALE DEFEDVTDTA 

       430        440        450        460        470        480 
GTAKTESNTS NDVKQESIND KTEDAVNATA TASGPSANAK PNDGDDDDDD DDDEMDIEFE 


DV 

References and documentation are available.

Number of amino acids: 482

Molecular weight: 54741.99

Theoretical pI: 4.39

Amino acid composition: 
Ala (A)  37	  7.7%
Arg (R)  22	  4.6%
Asn (N)  32	  6.6%
Asp (D)  51	 10.6%
Cys (C)   4	  0.8%
Gln (Q)  22	  4.6%
Glu (E)  60	 12.4%
Gly (G)  16	  3.3%
His (H)   7	  1.5%
Ile (I)  23	  4.8%
Leu (L)  39	  8.1%
Lys (K)  34	  7.1%
Met (M)  10	  2.1%
Phe (F)  11	  2.3%
Pro (P)  21	  4.4%
Ser (S)  28	  5.8%
Thr (T)  27	  5.6%
Trp (W)   2	  0.4%
Tyr (Y)  13	  2.7%
Val (V)  23	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 111
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2347
Hydrogen    H	      3710
Nitrogen    N	       652
Oxygen      O	       827
Sulfur      S	        14

Formula: C2347H3710N652O827S14
Total number of atoms: 7550

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30620
Abs 0.1% (=1 g/l)   0.559, assuming all pairs of Cys residues form cystines


Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.555, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.95
This classifies the protein as unstable.



Aliphatic index: 71.68

Grand average of hydropathicity (GRAVY): -0.948