ProtParam
User-provided sequence:
10 20 30 40 50 60
MDRSLGWQGN SVPEDRTEAG IKRFLEDTTD DGELSKFVKD FSGNASCHPP EAKTWASRPQ
70 80 90 100 110 120
VPEPRPQAPD LYDDDLEFRP PSRPQSSDNQ QYFCAPAPLS PSARPRSPWG KLDPYDSSED
130 140 150 160 170 180
DKEYVGFATL PNQVHRKSVK KGFDFTLMVA GESGLGKSTL VNSLFLTDLY RDRKLLGAEE
190 200 210 220 230 240
RIMQTVEITK HAVDIEEKGV RLRLTIVDTP GFGDAVNNTE CWKPVAEYID QQFEQYFRDE
250 260 270 280 290 300
SGLNRKNIQD NRVHCCLYFI SPFGHGLRPL DVEFMKALHQ RVNIVPILAK ADTLTPPEVD
310 320 330 340 350 360
HKKRKIREEI EHFGIKIYQF PDCDSDEDED FKLQDQALKE SIPFAVIGSN TVVEARGRRV
370 380 390 400 410 420
RGRLYPWGIV EVENPGHCDF VKLRTMLVRT HMQDLKDVTR ETHYENYRAQ CIQSMTRLVV
430 440 450 460 470
KERNRNKLTR ESGTDFPIPA VPPGTDPETE KLIREKDEEL RRMQEMLHKI QKQMKENY
References and
documentation are available.
Number of amino acids: 478
Molecular weight: 55098.16
Theoretical pI: 5.77
Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 70
Atomic composition:
Carbon C 2426
Hydrogen H 3804
Nitrogen N 692
Oxygen O 741
Sulfur S 18
Formula: C
2426H
3804N
692O
741S
18
Total number of atoms: 7681
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 47370
Abs 0.1% (=1 g/l) 0.860, assuming all pairs of Cys residues form cystines
Ext. coefficient 46870
Abs 0.1% (=1 g/l) 0.851, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.00
This classifies the protein as unstable.
Aliphatic index: 69.92
Grand average of hydropathicity (GRAVY): -0.786