ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDSASKEETL EKLVQEITVN LQKIDSNLSF CFHKITQDII PHVATYSEIC ERIMDSTEWL 

        70         80         90        100        110        120 
GTMFQETGLV NLQANAAAPV GNAPVKSLVS NNVGIFPTSA EEASRQSQTD NGPNEADSAV 

       130        140        150        160        170        180 
HVNRDVHSMF NNDSIDDFHT ANITSTGQIL KLPDSSDEDT GSEAVPSREQ TDLTGEGHGG 

       190        200        210        220        230        240 
ADDEQDESTI QRQSRKRKIS LLLQQQYGSS SSMVPSPIVP NKMRKQLAHE EHINNDGDND 

       250        260        270        280        290 
DENSNNIESS PLKQGHHHPK GQADDNNEGP DEEESTKEVP KPGTIIHFST NR 

References and documentation are available.

Number of amino acids: 292

Molecular weight: 32055.74

Theoretical pI: 4.63

Amino acid composition: 
Ala (A)  16	  5.5%
Arg (R)   9	  3.1%
Asn (N)  23	  7.9%
Asp (D)  24	  8.2%
Cys (C)   2	  0.7%
Gln (Q)  18	  6.2%
Glu (E)  26	  8.9%
Gly (G)  17	  5.8%
His (H)  12	  4.1%
Ile (I)  18	  6.2%
Leu (L)  16	  5.5%
Lys (K)  14	  4.8%
Met (M)   6	  2.1%
Phe (F)   7	  2.4%
Pro (P)  15	  5.1%
Ser (S)  32	 11.0%
Thr (T)  19	  6.5%
Trp (W)   1	  0.3%
Tyr (Y)   2	  0.7%
Val (V)  15	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1354
Hydrogen    H	      2139
Nitrogen    N	       399
Oxygen      O	       487
Sulfur      S	         8

Formula: C1354H2139N399O487S8
Total number of atoms: 4387

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     8605
Abs 0.1% (=1 g/l)   0.268, assuming all pairs of Cys residues form cystines


Ext. coefficient     8480
Abs 0.1% (=1 g/l)   0.265, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.76
This classifies the protein as unstable.



Aliphatic index: 65.79

Grand average of hydropathicity (GRAVY): -0.875