ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDSDLEPVAL TPQKQDSAWK HCEVYKYGDR VQMRCLYCRK MFKGGGITRV KEHLAGKKGQ 

        70         80         90        100        110        120 
GTICDQVPDE VRLFLQQCID GTVRRQRKRR KSSPEPLPIA YFPPCEVETQ VAASSDVNNG 

       130        140        150        160        170        180 
FKSPSSDVVV GQSTGRTKQR TYRSRKNNAF ERNDLANVEV DRDMDNLIPV AISSVKNIVH 

       190        200        210        220        230        240 
PTSKEREKTV HMAMGRFLFD IGADFDAANS VNVQPFIDAI VSGGFGVSIP THEDLRGWIL 

       250        260        270        280        290        300 
KSCVEEVKKE IDECKTLWKR TGCSVLVQEL NSNEGPLILK FLVYCPEKVV FLKSVDASEI 

       310        320        330        340        350        360 
LDSEDKLYEL LKEVVEEIGD TNVVQVITKC EDHYAAAGKK LMDVYPSLYW VPCAAHCIDK 

       370        380        390        400        410        420 
MLEEFGKMDW IREIIEQART VTRIIYNHSG VLNLMRKFTF GNDIVQPVCT SSATNFTTMG 

       430        440        450        460        470        480 
RIADLKPYLQ AMVTSSEWND CSYSKEAGGL AMTETINDED FWKALTLANH ITAPILRVLR 

       490        500        510        520        530        540 
IVCSERKPAM GYVYAAMYRA KEAIKTNLAH REEYIVYWKI IDRWWLQQPL YAAGFYLNPK 

       550        560        570        580        590        600 
FFYSIDEEMR SEIHLAVVDC IEKLVPDVNI QDIVIKDINS YKNAVGIFGR NLAIRARDTM 

       610        620        630        640        650        660 
LPAEWWSTYG ESCLNLSRFA IRILSQTCSS SIGSVRNLTS ISQIYESKNS IERQRLNDLV 

       670        680        690        700        710        720 
FVQYNMRLRR IGSESSGDDT VDPLSHSNME VLEDWVSRNQ VCIEGNGSSD WKSLEFIKRS 

       730        740        750        760 
EEVAVVIDET EDLGSGFDDA EIFKGEKEAS YEGGYSENLF T 

References and documentation are available.

Number of amino acids: 761

Molecular weight: 86390.39

Theoretical pI: 5.48

Amino acid composition: 
Ala (A)  46	  6.0%
Arg (R)  45	  5.9%
Asn (N)  35	  4.6%
Asp (D)  49	  6.4%
Cys (C)  20	  2.6%
Gln (Q)  25	  3.3%
Glu (E)  60	  7.9%
Gly (G)  42	  5.5%
His (H)  12	  1.6%
Ile (I)  53	  7.0%
Leu (L)  55	  7.2%
Lys (K)  48	  6.3%
Met (M)  19	  2.5%
Phe (F)  27	  3.5%
Pro (P)  26	  3.4%
Ser (S)  60	  7.9%
Thr (T)  35	  4.6%
Trp (W)  14	  1.8%
Tyr (Y)  27	  3.5%
Val (V)  63	  8.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 109
Total number of positively charged residues (Arg + Lys): 93

Atomic composition:

Carbon      C	      3821
Hydrogen    H	      6012
Nitrogen    N	      1042
Oxygen      O	      1162
Sulfur      S	        39

Formula: C3821H6012N1042O1162S39
Total number of atoms: 12076

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   118480
Abs 0.1% (=1 g/l)   1.371, assuming all pairs of Cys residues form cystines


Ext. coefficient   117230
Abs 0.1% (=1 g/l)   1.357, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.98
This classifies the protein as unstable.



Aliphatic index: 85.40

Grand average of hydropathicity (GRAVY): -0.317