ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK DSYVGDEAQS 

        70         80         90        100        110        120 
KRGILTLRYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPM NPKSNREKMT 

       130        140        150        160        170        180 
QIMFETFNVP AFYVSIQAVL SLYSSGRTTG IVLDSGDGVT HVVPIYAGFS LPHAILRIDL 

       190        200        210        220        230        240 
AGRDLTDYLM KILSERGYSF STTAEREIVR DIKEKLCYVA LDFEQEMQTA AQSSSIEKSY 

       250        260        270        280        290        300 
ELPDGQVITI GNERFRAPEA LFHPSVLGLE SAGIDQTTYN SIMKCDVDVR KELYGNIVMS 

       310        320        330        340        350        360 
GGTTMFPGIA ERMQKEITAL APSSMKVKII APPERKYSVW IGGSILASLT TFQQMWISKQ 

       370 
EYDESGPSIV HHKCF 

References and documentation are available.

Number of amino acids: 375

Molecular weight: 41689.63

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)  26	  6.9%
Arg (R)  18	  4.8%
Asn (N)   9	  2.4%
Asp (D)  20	  5.3%
Cys (C)   4	  1.1%
Gln (Q)  14	  3.7%
Glu (E)  27	  7.2%
Gly (G)  29	  7.7%
His (H)  10	  2.7%
Ile (I)  30	  8.0%
Leu (L)  25	  6.7%
Lys (K)  18	  4.8%
Met (M)  16	  4.3%
Phe (F)  14	  3.7%
Pro (P)  19	  5.1%
Ser (S)  31	  8.3%
Thr (T)  21	  5.6%
Trp (W)   4	  1.1%
Tyr (Y)  14	  3.7%
Val (V)  26	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1853
Hydrogen    H	      2906
Nitrogen    N	       494
Oxygen      O	       559
Sulfur      S	        20

Formula: C1853H2906N494O559S20
Total number of atoms: 5832

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43110
Abs 0.1% (=1 g/l)   1.034, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   1.028, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.04
This classifies the protein as unstable.



Aliphatic index: 84.24

Grand average of hydropathicity (GRAVY): -0.176