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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDSIDEQIAI KRKELQSLQK ITSLTDGLKI QLTELNEQIK EMGMNADSVA QLMNNWDSII

70 80 90 100 110 120
NNISQASLGL LQYAEGDYEI GPWKDSKKKE SEQSNETGLE AQENDKNDED NDEDEDLVPL

130
PETMVRIRVD GNE


References and documentation are available.

Number of amino acids: 133 Molecular weight: 15071.56 Theoretical pI: 4.13
Amino acid composition: 
Ala (A) 6 4.5% Arg (R) 3 2.3% Asn (N) 11 8.3% Asp (D) 14 10.5% Cys (C) 0 0.0% Gln (Q) 10 7.5% Glu (E) 17 12.8% Gly (G) 7 5.3% His (H) 0 0.0% Ile (I) 11 8.3% Leu (L) 13 9.8% Lys (K) 10 7.5% Met (M) 5 3.8% Phe (F) 0 0.0% Pro (P) 3 2.3% Ser (S) 10 7.5% Thr (T) 5 3.8% Trp (W) 2 1.5% Tyr (Y) 2 1.5% Val (V) 4 3.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 31 Total number of positively charged residues (Arg + Lys): 13 Atomic composition: Carbon C 639 Hydrogen H 1033 Nitrogen N 175 Oxygen O 234 Sulfur S 5 Formula: C639H1033N175O234S5 Total number of atoms: 2086 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 13980 Abs 0.1% (=1 g/l) 0.928 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 49.79 This classifies the protein as unstable. Aliphatic index: 83.61 Grand average of hydropathicity (GRAVY): -0.917