ProtParam
User-provided sequence:
10 20 30 40 50 60
MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV NKFKKSTNVE
70 80 90 100 110 120
ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK TTVGESVNGV QQPASSQSDA
130 140 150 160 170 180
MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD QVLKALYDVL AKESEHPPQS ILHTAKAIES
190 200 210 220 230 240
EMNKVNNCDT NEAAYKARYR IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP
250 260 270 280 290 300
LKQKIEEIAK QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE
ACGNRWKFS
References and
documentation are available.
Number of amino acids: 309
Molecular weight: 34843.47
Theoretical pI: 9.01
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 1516
Hydrogen H 2452
Nitrogen N 440
Oxygen O 478
Sulfur S 11
Formula: C
1516H
2452N
440O
478S
11
Total number of atoms: 4897
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24785
Abs 0.1% (=1 g/l) 0.711, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.701, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.78
This classifies the protein as unstable.
Aliphatic index: 73.24
Grand average of hydropathicity (GRAVY): -0.799