ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDSKHQCVKL NDGHFMPVLG FGTYAPAEVP KNKAIEATKL AIEAGFRHID SAHLYNNEEY 

        70         80         90        100        110        120 
VGLAIRSKIA DGTVKREDIF YTSKLWCNSH RPEFVRPALE RSLKNLQLDY VDLYLIHFPV 

       130        140        150        160        170        180 
SLKPGEELIP KDENGKLLFD TVDLCATWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP 

       190        200        210        220        230        240 
GLKYKPVCNQ VECHPYLNQR KLLDFCKSKD IVLVAYSALG SHREKPWVDQ NSPVLLEDPV 

       250        260        270        280        290        300 
LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN MKVFEFQLTS EDMKAIDGLD 

       310        320 
RNIRYLTLDI FAGPPNYPFS DEY 

References and documentation are available.

Number of amino acids: 323

Molecular weight: 36962.68

Theoretical pI: 8.29

Amino acid composition: 
Ala (A)  23	  7.1%
Arg (R)  17	  5.3%
Asn (N)  17	  5.3%
Asp (D)  20	  6.2%
Cys (C)   8	  2.5%
Gln (Q)  10	  3.1%
Glu (E)  22	  6.8%
Gly (G)  15	  4.6%
His (H)   9	  2.8%
Ile (I)  16	  5.0%
Leu (L)  38	 11.8%
Lys (K)  28	  8.7%
Met (M)   6	  1.9%
Phe (F)  13	  4.0%
Pro (P)  18	  5.6%
Ser (S)  16	  5.0%
Thr (T)   9	  2.8%
Trp (W)   3	  0.9%
Tyr (Y)  14	  4.3%
Val (V)  21	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1664
Hydrogen    H	      2620
Nitrogen    N	       450
Oxygen      O	       474
Sulfur      S	        14

Formula: C1664H2620N450O474S14
Total number of atoms: 5222

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37860
Abs 0.1% (=1 g/l)   1.024, assuming all pairs of Cys residues form cystines


Ext. coefficient    37360
Abs 0.1% (=1 g/l)   1.011, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.43
This classifies the protein as unstable.



Aliphatic index: 91.18

Grand average of hydropathicity (GRAVY): -0.362