ProtParam
User-provided sequence:
10 20 30 40 50 60
MDSLSKKIQN LGIHDIRNAA RFAQNVIVQY EPYQIDIRRA TNTDAWGPTP KHLAKVLRNR
70 80 90 100 110 120
YQVPLYLMTE YTLKRLVDHI ATRPKNLYEK ARKDYVNYGS EWRVVLKCLV VIEFLLLNVD
130 140 150 160 170 180
TGDELNQIRS CLLTHKHILT REIAQFKVKF SNDGKMEIHE RGIRKKGELI LQYLEDSQFL
190 200 210 220 230 240
KKERAKNKKN ALKIRQQGES SIYNANQIST SASYDNIDDD EFDADADGFD SEMDANNVTN
250 260 270 280 290 300
FNVPVETEAN SNTRRRSHME EQRRQRREIL REQIKNKEQQ RKRKQQQDSI PDLIDLDDST
310 320 330 340 350 360
STTNNITIDN GNNDNKNNNI NSNSDDDDDE FGDFQSETSP DTTAPKTSNS KIDDLLDWDG
370 380 390 400 410
PKSDTDTTAA AQTSLPFAEK KQQKARPQAT KDKSKGNDAF SDLFSYSKSL V
References and
documentation are available.
Number of amino acids: 411
Molecular weight: 47321.46
Theoretical pI: 6.07
Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 63
Atomic composition:
Carbon C 2044
Hydrogen H 3263
Nitrogen N 605
Oxygen O 674
Sulfur S 7
Formula: C
2044H
3263N
605O
674S
7
Total number of atoms: 6593
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34505
Abs 0.1% (=1 g/l) 0.729, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 0.727, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.56
This classifies the protein as unstable.
Aliphatic index: 71.46
Grand average of hydropathicity (GRAVY): -1.012