ProtParam
User-provided sequence:
10 20 30 40 50 60
MDSPVLQSAY DPSGQYLCYV TVALDKQRVG VQPTQRATSS GVDTVWNENF LYLEDSKLKV
70 80 90 100 110 120
TCLKWVNLAS SDTVAIILGM NNGEIWLYSV LANEVTYKFT TGNSYEIKDI DLMGNQLWCI
130 140 150 160 170 180
DSSDAFYQFD LLQFKLLQHF RINNCVQLNK LTIVPAGDSV AQLLVASHSI SLIDIEEKKV
190 200 210 220 230 240
VMTFPGHVSP VSTLQVITNE FFISGAEGDR FLNVYDIHSG MTKCVLVAES DIKELSHSGQ
250 260 270 280 290 300
ADSIAVTTED GSLEIFVDPL VSSSTKKRGN KSKKSSKKIQ IVSKDGRKVP IYNAFINKDL
310 320 330 340 350 360
LNVSWLQNAT MPYFKNLQWR EIPNEYTVEI SLNWNNKNKS ADRDLHGKDL ASATNYVEGN
370 380 390 400 410 420
ARVTSGDNFK HVDDAIKSWE RELTSLEQEQ AKPPQANELL TETFGDKLES STVARISGKK
430 440 450 460 470 480
TNLKGSNLKT ATTTGTVTVI LSQALQSNDH SLLETVLNNR DERVIRDTIF RLKPALAVIL
490 500 510 520 530 540
LERLAERIAR QTHRQGPLNV WVKWCLIIHG GYLVSIPNLM STLSSLHSTL KRRSDLLPRL
550 560 570 580 590 600
LALDARLDCT INKFKTLNYE AGDIHSSEPV VEEDEDDVEY NEELDDAGLI EDGEESYGSE
610 620 630 640
EEEEGDSDNE EEQKHTSSKQ DGRLETEQSD GEEEAGYSDV EME
References and
documentation are available.
Number of amino acids: 643
Molecular weight: 72001.62
Theoretical pI: 4.82
Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 3164
Hydrogen H 5011
Nitrogen N 857
Oxygen O 1029
Sulfur S 15
Formula: C
3164H
5011N
857O
1029S
15
Total number of atoms: 10076
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 82195
Abs 0.1% (=1 g/l) 1.142, assuming all pairs of Cys residues form cystines
Ext. coefficient 81820
Abs 0.1% (=1 g/l) 1.136, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.77
This classifies the protein as unstable.
Aliphatic index: 90.33
Grand average of hydropathicity (GRAVY): -0.424