ProtParam
User-provided sequence:
10 20 30 40 50 60
MDSQPVDVDN IIDRLLEVRG SKPGQQVDLE ENEIRYLCSK ARSIFIKQPI LLELEAPIKI
70 80 90 100 110 120
CGDIHGQYYD LLRLFEYGGF PPESNYLFLG DYVDRGKQSL ETICLLLAYK IKYPENFFIL
130 140 150 160 170 180
RGNHECASIN RIYGFYDECK RRYNIKLWKT FTDCFNCLPI AAIIDEKIFC MHGGLSPDLN
190 200 210 220 230 240
SMEQIRRVMR PTDIPDVGLL CDLLWSDPDK DIVGWSENDR GVSFTFGPDV VNRFLQKQDM
250 260 270 280 290 300
ELICRAHQVV EDGYEFFSKR QLVTLFSAPN YCGEFDNAGA MMSVDESLLC SFQILKPAQK
310
SLPRQAGGRK KK
References and
documentation are available.
Number of amino acids: 312
Molecular weight: 35907.29
Theoretical pI: 5.31
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1610
Hydrogen H 2502
Nitrogen N 426
Oxygen O 467
Sulfur S 19
Formula: C
1610H
2502N
426O
467S
19
Total number of atoms: 5024
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36620
Abs 0.1% (=1 g/l) 1.020, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.999, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.55
This classifies the protein as unstable.
Aliphatic index: 89.04
Grand average of hydropathicity (GRAVY): -0.272