ProtParam
User-provided sequence:
10 20 30 40 50 60
MDSQVEGKIS PSQKESSSTS GLVSPSEDGP AHQKIHRDQL SVDQIKKIRE ERAQKRQVRR
70 80 90 100 110 120
NSLISQGKDP DFPTPDLQFI ERPFLPINHD NSKGLTPATI QVTQDSLDVK IMTYNTLAQT
130 140 150 160 170 180
LIRRDFFPES GPALKWHKRS KVLVHELKKY RPDVVSLQEV DYNELNFWQE NFHKLGFDVI
190 200 210 220 230 240
FKRHEGKTHG LLVAWNNKKF QLDNDWMLDY DNILAGNVIS ARTRTKNIAL IISLYFKGIT
250 260 270 280 290 300
DSSSRGIIVA NTHLFWHPFG VFERLRQSYL VLQKIQEIKA CSKYNGWHSL LMGDFNTEPE
310 320 330 340 350 360
EPPYLAITKR PLILKGPIRA MVECSLAYRY SKKRNGEESD QDDEECDEKS RGEGHSDQPQ
370 380 390 400 410 420
NPKPESFTAT KEEKALVNQL VALHNSLHVK GVSLYGIGYG KVHPENANGS HGEPGLSNWA
430 440 450 460 470 480
NTWCGLLDYI FYIEGDHNQD TRQKEPLNAF EGNNNVKIIG YLRMPCAQEM PKHSQPFEGE
490 500
YASDHISLMC QIRLFFGGEK VHSLK
References and
documentation are available.
Number of amino acids: 505
Molecular weight: 57742.26
Theoretical pI: 7.06
Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 2571
Hydrogen H 3999
Nitrogen N 723
Oxygen O 766
Sulfur S 14
Formula: C
2571H
3999N
723O
766S
14
Total number of atoms: 8073
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 68215
Abs 0.1% (=1 g/l) 1.181, assuming all pairs of Cys residues form cystines
Ext. coefficient 67840
Abs 0.1% (=1 g/l) 1.175, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.61
This classifies the protein as unstable.
Aliphatic index: 77.41
Grand average of hydropathicity (GRAVY): -0.667