ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDSSLKTQEP QVVETSPSPV AQEPPQVADK PAIVPSPIAQ EPDNDVPAPG NEFAEFAAGC 

        70         80         90        100        110        120 
FWGVELAFQR IPGVTVTEVG YTHGISHNPS YEDVCTNTTN HAEVVRVQYD PKECTYETLL 

       130        140        150        160        170        180 
DLFWSRHNPT TLNRQGELLG AQYRSGIYFY TPEQEKLARE SLEKEQKKLE DKIVTEILPA 

       190        200        210 
KKFYKAEEYH QQYLVKGGMH GNAQSPAKSC KDPIRCYG 

References and documentation are available.

Number of amino acids: 218

Molecular weight: 24434.32

Theoretical pI: 5.11

Amino acid composition: 
Ala (A)  16	  7.3%
Arg (R)   7	  3.2%
Asn (N)   8	  3.7%
Asp (D)   9	  4.1%
Cys (C)   5	  2.3%
Gln (Q)  14	  6.4%
Glu (E)  22	 10.1%
Gly (G)  13	  6.0%
His (H)   6	  2.8%
Ile (I)   8	  3.7%
Leu (L)  13	  6.0%
Lys (K)  14	  6.4%
Met (M)   2	  0.9%
Phe (F)   7	  3.2%
Pro (P)  19	  8.7%
Ser (S)  12	  5.5%
Thr (T)  14	  6.4%
Trp (W)   2	  0.9%
Tyr (Y)  11	  5.0%
Val (V)  16	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	      1086
Hydrogen    H	      1665
Nitrogen    N	       289
Oxygen      O	       340
Sulfur      S	         7

Formula: C1086H1665N289O340S7
Total number of atoms: 3387

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27640
Abs 0.1% (=1 g/l)   1.131, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   1.121, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.28
This classifies the protein as unstable.



Aliphatic index: 66.19

Grand average of hydropathicity (GRAVY): -0.663