ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDSSNLSSSM PQEGQLKLTE EAIITIKQVD ESSAKRSVSN ESRKSKDVTR KEQIASDQQG 

        70         80         90        100        110        120 
DDQCPQSDTA KDKKTEFKQD EVPNSNGKSI PTFHPCNNIG INDFNHQHYS RVCRNGIDIN 

       130        140        150        160        170        180 
LIVVGESSLG KTTFVNSFLQ SNDTNFRPKK TMDFVEHKAT LSDGDQKFNL TIVDTPGFGD 

       190        200        210        220        230        240 
KSDNSNCWRP IATNLLHRLN AYFQNEVKMD RETSEIDSRI HGCLFFINPN GHRLQPLEIY 

       250        260        270        280        290        300 
IMKKIDQFVN IIPVIGKADT MTSDELNHFK KRVIADMVRE KIRYFREPHN EKKAKIPIPF 

       310        320        330        340        350        360 
AIVGAGAPIE HDGKCIRGRA YPWGLVDIDD PKQSDFCQLR NFLLYTHIEG LKHKTHKLIY 

       370        380        390        400        410        420 
DTFRTEKLVA LNATPGSQFI SAEEMNQKYI SEQTQLVEEA LTKVMKEKYR EKENNLELLE 

       430        440        450        460 
TNLKTHHKDY KHALKKRITA LEEEKNRLIK EIGPEKVKKA GILA 

References and documentation are available.

Number of amino acids: 464

Molecular weight: 53104.26

Theoretical pI: 8.27

Amino acid composition: 
Ala (A)  22	  4.7%
Arg (R)  22	  4.7%
Asn (N)  31	  6.7%
Asp (D)  31	  6.7%
Cys (C)   7	  1.5%
Gln (Q)  21	  4.5%
Glu (E)  36	  7.8%
Gly (G)  22	  4.7%
His (H)  16	  3.4%
Ile (I)  37	  8.0%
Leu (L)  33	  7.1%
Lys (K)  48	 10.3%
Met (M)   9	  1.9%
Phe (F)  20	  4.3%
Pro (P)  19	  4.1%
Ser (S)  30	  6.5%
Thr (T)  27	  5.8%
Trp (W)   2	  0.4%
Tyr (Y)  10	  2.2%
Val (V)  21	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      2335
Hydrogen    H	      3728
Nitrogen    N	       664
Oxygen      O	       718
Sulfur      S	        16

Formula: C2335H3728N664O718S16
Total number of atoms: 7461

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26275
Abs 0.1% (=1 g/l)   0.495, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.488, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.93
This classifies the protein as stable.



Aliphatic index: 76.70

Grand average of hydropathicity (GRAVY): -0.734