ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDSTGASQIV SALDVIYSPK SNNSQRQEAQ KFLDEVKLCS ESPFWGYEIA LQNPTNSILK 

        70         80         90        100        110        120 
YFGLGLLDHA VKKNWNDYDE GKRVALRKWV MELNFGVQDY DTRYIKEKLA TLWVEVAKRT 

       130        140        150        160        170        180 
WGEALKQTNP TEEQLLTSWV DMDNNLFELW NINQSSRELA LIIFRILFED VFLLDDLIVL 

       190        200        210        220        230        240 
KRMTVIQPLC VMIVCPIEVF AIKYKFSDKW TKFKANEEGW FSVWIPELNN ALQQNNSEYI 

       250        260        270        280        290        300 
IRLLETLKTC LNWPLTEVIV RNDVLSSLLT CLSSNIPRAQ SMALDSIHIL LTRPYSNESH 

       310        320        330        340        350        360 
YQMTIDRVFD NMDLLDSVYE SLLFDPTDDI DETKYPIIKK FVDMISCLYV CVPKIKETNG 

       370        380        390        400        410        420 
QIQKYFKLVL KTTYNPSLIV SGLTLDLWCT CLRNDEYLPK LEKYVIPDLL QFAADALVYY 

       430        440        450        460        470        480 
EQIDGHISKK FAEIDFQSKS EFQTFCSTYR KRIRDIIRLI SCVELDLTYD WLNNRLNNYF 

       490        500        510        520        530        540 
SSPFGQQVLS STFLDHKLEP YLGALSQYMI VECFINGCIR WKIWYPTGDD YDEKLDSILQ 

       550        560        570        580        590        600 
KLEILSNQLI ALNLREPLLL KKQIQNFALF LTMLKDNVLF TLLEKIITSA TMDYPEINLE 

       610        620        630        640        650        660 
ERGAESDAVR DLRYACGIEL NRMALLMPES LKKIYPDLES VIARIMPNLS YHEKISFKSF 

       670        680        690        700        710        720 
LLIIVLKSSL DMKEERFAAI VDPELLAWSD KTTVVGLSDL HWFMERLGIV QIAEYFQRRD 

       730        740        750        760        770        780 
IDENSDLLSI PIDDEGKELK SELTKRWQSL FPVRATRMFI HYSMQSIKTD EEFKMLQDLW 

       790        800        810        820        830        840 
RPRIVPILPY ITRLLYQLQS YHDPDNWKGL PTVVQSFVKY STIERFWEAG ASNKSKDEFI 

       850        860        870        880        890        900 
DEHMKAMQTL RDFADSVGHI IRYTREYTLL VLSAISSLGS VFYLLDESPD LLLNSIAIFK 

       910        920        930        940        950        960 
PGSNEISPGV STHGWKHIMN IAIRPILKGC PKDCLGKFMP AFLPKLFEIL DLLLCQKWSS 

       970        980        990       1000       1010       1020 
HMNDMDMNPV PTDDDQMTEE ILEENLLRQL TTVVVRIVID CVGQGNANPN SAKSRLNNHQ 

      1030       1040       1050       1060       1070       1080 
MEMRKIIFND LNTLAPFLKL LNHLISFKDT KCSFNSILVM KCCLTSVLNQ NNTVDEYFTF 

      1090       1100       1110       1120       1130       1140 
EVMKNLLLNV LCNSAFKDSF HEALYAFTVI FLTLCKEYPS ARAFLFEISN GYNIDELYRN 

      1150       1160       1170       1180       1190       1200 
LRSVDEYKTQ RALMIDFIDW VKSTSGKEDG NVDHAGDERK RQEKREAILK KANERLIKKN 

      1210       1220 
KENGDMLDDP NIEDGAVGNL FDDN 

References and documentation are available.

Number of amino acids: 1224

Molecular weight: 142117.88

Theoretical pI: 5.19

Amino acid composition: 
Ala (A)  54	  4.4%
Arg (R)  53	  4.3%
Asn (N)  71	  5.8%
Asp (D)  88	  7.2%
Cys (C)  23	  1.9%
Gln (Q)  44	  3.6%
Glu (E)  83	  6.8%
Gly (G)  38	  3.1%
His (H)  18	  1.5%
Ile (I)  94	  7.7%
Leu (L) 158	 12.9%
Lys (K)  84	  6.9%
Met (M)  34	  2.8%
Phe (F)  59	  4.8%
Pro (P)  45	  3.7%
Ser (S)  87	  7.1%
Thr (T)  57	  4.7%
Trp (W)  24	  2.0%
Tyr (Y)  45	  3.7%
Val (V)  65	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 171
Total number of positively charged residues (Arg + Lys): 137

Atomic composition:

Carbon      C	      6429
Hydrogen    H	     10058
Nitrogen    N	      1642
Oxygen      O	      1871
Sulfur      S	        57

Formula: C6429H10058N1642O1871S57
Total number of atoms: 20057

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   200425
Abs 0.1% (=1 g/l)   1.410, assuming all pairs of Cys residues form cystines


Ext. coefficient   199050
Abs 0.1% (=1 g/l)   1.401, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.43
This classifies the protein as unstable.



Aliphatic index: 100.11

Grand average of hydropathicity (GRAVY): -0.180