ProtParam
User-provided sequence:
10 20 30 40 50 60
MDSVTNFFWN DTYNAGTPTR STLKGKKVQN GIDGKSQAKK ESISSGSRTS DPTRGSLPSS
70 80 90 100 110 120
SGQPTSGGGF PSTSNIQKMM ADTLVEKIIK MALPPSSKTA VDTIHHRMVA GKERPKLSVQ
130 140 150 160 170 180
ITSRNFIQMN SRLGVPFMIM DELIKILNWT NPAYTVSIMF LYTLIILKPF QMLSSLPIFY
190 200 210 220 230 240
LLFCVMVPQY LYIHKPNPTS YLDNNQTPAQ GPPLRRPEVP KPVPELSQEF VLNLTDLQNH
250 260 270 280 290 300
MLLYVKFYDF TLLILQKFAF FTNEAISSFY FIVLLIIATL NFLYMDKFIK LIPMRPVLIL
310 320 330 340 350 360
LGWGFFIASH PSNREYLLTK LNSEETRLKT LTISTNLESK ILQHLKLIEA REHRLVMIFE
370 380 390 400 410 420
IQKYLPEYKE WRPVGFSDDD YSLFSSLRIY QRRIEENSVK SLEEIEPPKD WEWEANSHWV
430 440 450 460 470 480
LDLDPKEWVE DEFIQYVEID SETKWVYDLN LDGQRGSYRR RMWTNSCVRK KLDSGISSNL
490 500 510 520 530 540
GEEEVVNPLR EETYRQGVHG VTKGSMSGGL THSSDDDRAD EESINGTIPN LNNIDADASY
550
PSIEELTDTL NSTI
References and
documentation are available.
Number of amino acids: 554
Molecular weight: 63542.68
Theoretical pI: 5.81
Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 2867
Hydrogen H 4474
Nitrogen N 744
Oxygen O 848
Sulfur S 19
Formula: C
2867H
4474N
744O
848S
19
Total number of atoms: 8952
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 86415
Abs 0.1% (=1 g/l) 1.360, assuming all pairs of Cys residues form cystines
Ext. coefficient 86290
Abs 0.1% (=1 g/l) 1.358, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.94
This classifies the protein as unstable.
Aliphatic index: 88.83
Grand average of hydropathicity (GRAVY): -0.330