ProtParam
User-provided sequence:
10 20 30 40 50 60
MDTDLDVPMQ DAVTEQLTPT VSEDMDLNNN SSDNNAEEFS VDDLKPGSSG IADHKSSKPL
70 80 90 100 110 120
ELNNTNINQL DQWIEHLSKC EPLSEDDVAR LCKMAVDVLQ FEENVKPINV PVTICGDVHG
130 140 150 160 170 180
QFHDLLELFK IGGPCPDTNY LFMGDYVDRG YYSVETVSYL VAMKVRYPHR ITILRGNHES
190 200 210 220 230 240
RQITQVYGFY DECLRKYGSA NVWKMFTDLF DYFPITALVD NKIFCLHGGL SPMIETIDQV
250 260 270 280 290 300
RELNRIQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGFTF GQDVSEQFNH TNDLSLIARA
310 320 330 340 350 360
HQLVMEGYAW SHQQNVVTIF SAPNYCYRCG NQAAIMEVDE NHNRQFLQYD PSVRPGEPSV
SRKTPDYFL
References and
documentation are available.
Number of amino acids: 369
Molecular weight: 41937.87
Theoretical pI: 4.61
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1849
Hydrogen H 2813
Nitrogen N 499
Oxygen O 579
Sulfur S 20
Formula: C
1849H
2813N
499O
579S
20
Total number of atoms: 5760
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 50350
Abs 0.1% (=1 g/l) 1.201, assuming all pairs of Cys residues form cystines
Ext. coefficient 49850
Abs 0.1% (=1 g/l) 1.189, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.56
This classifies the protein as stable.
Aliphatic index: 76.80
Grand average of hydropathicity (GRAVY): -0.452