ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDTDLDVPMQ DAVTEQLTPT VSEDMDLNNN SSDNNAEEFS VDDLKPGSSG IADHKSSKPL 

        70         80         90        100        110        120 
ELNNTNINQL DQWIEHLSKC EPLSEDDVAR LCKMAVDVLQ FEENVKPINV PVTICGDVHG 

       130        140        150        160        170        180 
QFHDLLELFK IGGPCPDTNY LFMGDYVDRG YYSVETVSYL VAMKVRYPHR ITILRGNHES 

       190        200        210        220        230        240 
RQITQVYGFY DECLRKYGSA NVWKMFTDLF DYFPITALVD NKIFCLHGGL SPMIETIDQV 

       250        260        270        280        290        300 
RELNRIQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGFTF GQDVSEQFNH TNDLSLIARA 

       310        320        330        340        350        360 
HQLVMEGYAW SHQQNVVTIF SAPNYCYRCG NQAAIMEVDE NHNRQFLQYD PSVRPGEPSV 


SRKTPDYFL 

References and documentation are available.

Number of amino acids: 369

Molecular weight: 41937.87

Theoretical pI: 4.61

Amino acid composition: 
Ala (A)  15	  4.1%
Arg (R)  16	  4.3%
Asn (N)  23	  6.2%
Asp (D)  33	  8.9%
Cys (C)   9	  2.4%
Gln (Q)  18	  4.9%
Glu (E)  23	  6.2%
Gly (G)  23	  6.2%
His (H)  12	  3.3%
Ile (I)  18	  4.9%
Leu (L)  30	  8.1%
Lys (K)  12	  3.3%
Met (M)  11	  3.0%
Phe (F)  16	  4.3%
Pro (P)  21	  5.7%
Ser (S)  24	  6.5%
Thr (T)  17	  4.6%
Trp (W)   5	  1.4%
Tyr (Y)  15	  4.1%
Val (V)  28	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1849
Hydrogen    H	      2813
Nitrogen    N	       499
Oxygen      O	       579
Sulfur      S	        20

Formula: C1849H2813N499O579S20
Total number of atoms: 5760

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50350
Abs 0.1% (=1 g/l)   1.201, assuming all pairs of Cys residues form cystines


Ext. coefficient    49850
Abs 0.1% (=1 g/l)   1.189, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.56
This classifies the protein as stable.



Aliphatic index: 76.80

Grand average of hydropathicity (GRAVY): -0.452