ProtParam
User-provided sequence:
10 20 30 40 50 60
MDTEADLFYR QLPKVELHAH LNGSVSFETM EKLIKRKPHL NIEHSMTAIR RGQRRTLDEC
70 80 90 100 110 120
FQVFKVIHQL VDSEEDILMV AKSVIQEFAA DGVKYLELRS TPREVTETGL SKQRYIETVL
130 140 150 160 170 180
EAIRQCKQEG VDIDVRFLVA VDRRHGPEVA MQTVKLAEDF LLSSDGTVVG LDLSGDPTVG
190 200 210 220 230 240
HGKDLLAALQ KAKNCGLKLA LHLSEVPSQI DETELLLNLP PDRIGHGTFL HPDVGGSDSL
250 260 270 280 290 300
VDKVCKQNIP IEICLTSNVK GQTVPSYDKH HFKYWYNRGH PCVLCTDDKG VFCTDLSQEY
310 320 330 340
QLAASTFGLT KEAVWILSQQ AIGYTFAPEP IKQRLEKTWA ELKQQILQ
References and
documentation are available.
Number of amino acids: 348
Molecular weight: 39189.89
Theoretical pI: 5.83
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1742
Hydrogen H 2779
Nitrogen N 477
Oxygen O 523
Sulfur S 13
Formula: C
1742H
2779N
477O
523S
13
Total number of atoms: 5534
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28920
Abs 0.1% (=1 g/l) 0.738, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.725, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.77
This classifies the protein as stable.
Aliphatic index: 95.20
Grand average of hydropathicity (GRAVY): -0.279