ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDTSHEIHDK IPDTLREQQQ HLRQKESEGC ITTLKDLNVP ETKKLSSVLH GRKASTYLRI 

        70         80         90        100        110        120 
FRDDECLADN NNGVDSNNGG SVTCADKITR SEATPKSVPE GLQVSEKKNN PDTLSSSLSS 

       130        140        150        160        170        180 
FILSNHEEPA IKPNKHVAHR NNITETGQGS GEDIAKQQSH QPQVLHHQTS LKPIQNVDEG 

       190        200        210        220        230        240 
CISPKSTYQE SLHGISEDLT LKPVSSATYY PHKSKADSGY EEKDKMENDI DTIQPATINC 

       250        260        270        280        290        300 
ASGIATLPSS YNRHTFKVKT YSTLSQSLRQ ENVNNRSNEK KPQQFVPHSE SIKEKPNTFE 

       310        320        330        340        350        360 
QDKEGEQADE EEDEGDNEHR EYPLAVELKP FTNRVGGHTA IFRFSKRAVC KALVNRENRW 

       370        380        390        400        410        420 
YENIELCHKE LLQFMPRYIG VLNVRQHFQS KDDFLSDLDQ ENNGKNDTSN ENKDIEVNHN 

       430        440        450        460        470        480 
NNDDIALNTE PTGTPLTHIH SFPLEHSSRQ VLEKEHPEIE SVHPHVKRSL SSSNQPSLLP 

       490        500        510        520        530        540 
EVVLNDNRHI IPESLWYKYS DSPNSAPNDS YFSSSSSHNS CSFGERGNTN KLKRRDSGST 

       550        560        570        580        590        600 
MINTELKNLV IREVFAPKCF RRKRNSNTTT MGNHNARLGS SPSFLTQKSR ASSHDASNTS 

       610        620        630        640        650        660 
MKTLGDSSSQ ASLQMDDSKV NPNLQDPFLK KSLHEKISNA LDGSHSVMDL KQFHKNEQIK 

       670        680        690        700        710        720 
HKNSFCNSLS PILTATNSRD DGEFATSPNY ISNAQDGVFD MDEDTGNETI NMDNHGCHLD 

       730        740        750        760        770        780 
SGKNMIIKSL AYNVSNDYSH HDIESITFEE TSHTIVSKFI LLEDLTRNMN KPCALDLKMG 

       790        800        810        820        830        840 
TRQYGVDAKR AKQLSQRAKC LKTTSRRLGV RICGLKVWNK DYYITRDKYF GRRVKVGWQF 

       850        860        870        880        890        900 
ARVLARFLYD GKTIESLIRQ IPRLIKQLDT LYSEIFNLKG YRLYGASLLL MYDGDANKSN 

       910        920        930        940        950        960 
SKRKKAANVK VNLIDFARCV TKEDAMECMD KFRIPPKSPN IEDKGFLRGV KSLRFYLLLI 

       970        980        990       1000       1010       1020 
WNYLTSDMPL IFDEVEMNDM ISEEADSNSF TSATGSKINF NSKWDWLDEF DKEDEEMYND 

      1030       1040       1050 
PNSKLRQKWR KYELIFDAEP RYNDDAQVSD 

References and documentation are available.

Number of amino acids: 1050

Molecular weight: 119550.05

Theoretical pI: 6.32

Amino acid composition: 
Ala (A)  45	  4.3%
Arg (R)  54	  5.1%
Asn (N)  84	  8.0%
Asp (D)  76	  7.2%
Cys (C)  16	  1.5%
Gln (Q)  41	  3.9%
Glu (E)  75	  7.1%
Gly (G)  45	  4.3%
His (H)  38	  3.6%
Ile (I)  54	  5.1%
Leu (L)  85	  8.1%
Lys (K)  84	  8.0%
Met (M)  20	  1.9%
Phe (F)  37	  3.5%
Pro (P)  44	  4.2%
Ser (S) 112	 10.7%
Thr (T)  58	  5.5%
Trp (W)   8	  0.8%
Tyr (Y)  30	  2.9%
Val (V)  44	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 151
Total number of positively charged residues (Arg + Lys): 138

Atomic composition:

Carbon      C	      5182
Hydrogen    H	      8163
Nitrogen    N	      1505
Oxygen      O	      1678
Sulfur      S	        36

Formula: C5182H8163N1505O1678S36
Total number of atoms: 16564

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    89700
Abs 0.1% (=1 g/l)   0.750, assuming all pairs of Cys residues form cystines


Ext. coefficient    88700
Abs 0.1% (=1 g/l)   0.742, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.28
This classifies the protein as unstable.



Aliphatic index: 68.07

Grand average of hydropathicity (GRAVY): -0.866