ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDTSKKDTTR SPSHSNSSSP SSSSLSSSSS KEKKRPKRLS SQNVNYDLKR RKIITSEGIE 

        70         80         90        100        110        120 
RSFKNEHSNL AVEDNIPEEE PKELLEKDSK GNIIKLNEPS TISEDSKVSV TGLPLNKGPS 

       130        140        150        160        170        180 
EKIKRESLWN YRKNLGGQSN NSEMTLVPSK RFTQVPKNFQ DLNRNDLKTF LTENMTEESN 

       190        200        210        220        230        240 
IRSTIGWNGD IINRTRDREP ESDRDNKKLS NIRTKIILST NATYDSKSKL FGQNSIKSTS 

       250        260        270        280        290        300 
NASEKIFRDK NNSTIDFENE DFCSACNQSG SFLCCDTCPK SFHFLCLDPP IDPNNLPKGD 

       310        320        330        340        350        360 
WHCNECKFKI FINNSMATLK KIESNFIKQN NNVKIFAKLL FNIDSHNPKQ FQLPNYIKET 

       370        380        390        400        410        420 
FPAVKTGSRG QYSDENDKIP LTDRQLFNTS YGQSITKLDS YNPDTHIDSN SGKFLICYKC 

       430        440        450        460        470        480 
NQTRLGSWSH PENSRLIMTC DYCQTPWHLD CVPRASFKNL GSKWKCPLHS PTKVYKKIHH 

       490        500        510        520        530        540 
CQEDNSVNYK VWKKQRLINK KNQLYYEPLQ KIGYQNNGNI QIIPTTSHTD YDFNQDFKIT 

       550        560        570        580        590        600 
QIDENSIKYD FFDKIYKSKM VQKRKLFQFQ ESLIDKLVSN GSQNGNSEDN MVKDIASLIY 

       610        620        630        640        650        660 
FQVSNNDKSS NNKSASKSNN LRKLWDLKEL TNVVVPNELD SIQFNDFSSD EIKHLLYLKK 

       670        680 
IIESKPKEEL LKFLNIENPE NQSE 

References and documentation are available.

Number of amino acids: 684

Molecular weight: 78836.46

Theoretical pI: 8.95

Amino acid composition: 
Ala (A)  10	  1.5%
Arg (R)  25	  3.7%
Asn (N)  73	 10.7%
Asp (D)  44	  6.4%
Cys (C)  15	  2.2%
Gln (Q)  29	  4.2%
Glu (E)  42	  6.1%
Gly (G)  21	  3.1%
His (H)  13	  1.9%
Ile (I)  49	  7.2%
Leu (L)  53	  7.7%
Lys (K)  77	 11.3%
Met (M)   7	  1.0%
Phe (F)  31	  4.5%
Pro (P)  31	  4.5%
Ser (S)  83	 12.1%
Thr (T)  35	  5.1%
Trp (W)   8	  1.2%
Tyr (Y)  19	  2.8%
Val (V)  19	  2.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 102

Atomic composition:

Carbon      C	      3454
Hydrogen    H	      5452
Nitrogen    N	       972
Oxygen      O	      1096
Sulfur      S	        22

Formula: C3454H5452N972O1096S22
Total number of atoms: 10996

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73185
Abs 0.1% (=1 g/l)   0.928, assuming all pairs of Cys residues form cystines


Ext. coefficient    72310
Abs 0.1% (=1 g/l)   0.917, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.79
This classifies the protein as unstable.



Aliphatic index: 67.68

Grand average of hydropathicity (GRAVY): -0.930