ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDTSMPNQPK FLPRIEHNSS GATANSYVAT ASGNPFETDE EDEIFSRHKM VLRVPSNCTG 

        70         80         90        100        110        120 
DLMHLAVSRN WLVCLLGTPE RTTLLRFFLP RAIPPGEAVL EKYLSGSGYK ITRMFLDPTG 

       130        140        150        160        170        180 
HHIIIALVPK SATAGVSPDF LYIHCLESPQ AQQLKVRRIE KFKDHEITAV AFNPYHGNES 

       190        200        210        220        230        240 
STGPILLGTS RGLIFETELN PAADGHVQRK QLYDLGLGRP KYPITGLKLL RVPNSSRYII 

       250        260        270        280        290        300 
VVTSPECIYT FQETLKAEER SLQAIFAGYV SGVQEPHCEE RKTDLTFSQL RFFAPPNSKY 

       310        320        330        340        350        360 
PKQWAWLCGE GIRVGELSIE ANSAATLIGN TLINLDFEKT MHLSYGERRL NTPKAFVLTE 

       370        380        390        400        410        420 
YHAVLLYADH VRAICLLNQE QVYQEAFDEA RVGKPLSIER DELTGSIYVY TVKTVFNLRV 

       430        440        450        460        470        480 
TREERNVWRI YLDKGQYELA TAHAAEDPEH LQLVLCQRAD AAFADGSYQV AADYYAETDK 

       490        500        510        520        530        540 
SFEEVCLKFM VLPDKRPIIN YVKKRLSRVT TKPMETDELD EDKMNIIKAL VIWLIDLYLI 

       550        560        570        580        590        600 
QINMPDKDEE WRSSWQTEYD EFMMEAHVLS CTRQNRETVR QLIAEHADPR NMAQFAIAIG 

       610        620        630        640        650        660 
DYDEVVAQQL KAECYAEALQ TLINQRNPEL FYKYAPELIT RLPKPTVDAL MAQGSRLEVE 

       670        680        690        700        710        720 
KLVPTLIIME NREQREQTQR YLEFAIYKLN TTNDAIHNFL LHLYAEHEPK LLMKYLEIQG 

       730        740        750        760        770        780 
RDESLVHYDI YYAHKVCTDL DVKEARVFLE CMLRKWISAV DLALTFDMKL AKETASRPSD 

       790        800        810        820        830        840 
SKIRRKLWLR IAYHDIKGTN DVKKALNLLK ECDLLRIEDL LPFFADFEKI DNFKEAICDA 

       850        860        870        880        890        900 
LRDYNQRIQE LQREMAETTE QTDRVTAELQ QLRQHSLTVE SQDTCEICEM MLLVKPFFIF 

       910        920        930        940        950        960 
ICGHKFHSDC LEKHVVPLLT KEQCRRLGTL KQQLEAEVQT QAQPQSGALS KQQAMELQRK 

       970        980        990       1000 
RAALKTEIED ILAADCLFCG LLISTIDQPF VDDWEQVNVE WE 

References and documentation are available.

Number of amino acids: 1002

Molecular weight: 115334.11

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  78	  7.8%
Arg (R)  60	  6.0%
Asn (N)  34	  3.4%
Asp (D)  56	  5.6%
Cys (C)  22	  2.2%
Gln (Q)  52	  5.2%
Glu (E)  90	  9.0%
Gly (G)  36	  3.6%
His (H)  27	  2.7%
Ile (I)  59	  5.9%
Leu (L) 115	 11.5%
Lys (K)  57	  5.7%
Met (M)  22	  2.2%
Phe (F)  39	  3.9%
Pro (P)  44	  4.4%
Ser (S)  46	  4.6%
Thr (T)  60	  6.0%
Trp (W)  11	  1.1%
Tyr (Y)  38	  3.8%
Val (V)  56	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 146
Total number of positively charged residues (Arg + Lys): 117

Atomic composition:

Carbon      C	      5152
Hydrogen    H	      8110
Nitrogen    N	      1390
Oxygen      O	      1525
Sulfur      S	        44

Formula: C5152H8110N1390O1525S44
Total number of atoms: 16221

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   118495
Abs 0.1% (=1 g/l)   1.027, assuming all pairs of Cys residues form cystines


Ext. coefficient   117120
Abs 0.1% (=1 g/l)   1.015, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.19
This classifies the protein as unstable.



Aliphatic index: 91.72

Grand average of hydropathicity (GRAVY): -0.329