ProtParam
User-provided sequence:
10 20 30 40 50 60
MDTTEELKIS HYKLYKCFDF LVVANNKIEV AQAFVKYRRP EYFLPLVGVE FVSLTLVGKH
70 80 90 100 110 120
FKLGKYSVVV LRGEVHSETL RCLLPKLTDQ PIGSSFLLLR PFRDVGNYCL LLHILPSSKK
130 140 150 160 170 180
FTLAMQFLCE LIDSTSKEEE DVFRVLVYAS DELLDVKTYR ALIGCSWELF RCSPGEGCNC
190 200 210 220 230 240
PDMCRLQHLI EDSTLVKVGI GIDGDSVKLF HDYGLLKPNR IRLGNWEVHP LSKQQLQYAA
250 260
TDAYASWHLH QVLKDLPDAV SGS
References and
documentation are available.
Number of amino acids: 263
Molecular weight: 29948.79
Theoretical pI: 6.50
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1363
Hydrogen H 2134
Nitrogen N 352
Oxygen O 382
Sulfur S 12
Formula: C
1363H
2134N
352O
382S
12
Total number of atoms: 4243
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33390
Abs 0.1% (=1 g/l) 1.115, assuming all pairs of Cys residues form cystines
Ext. coefficient 32890
Abs 0.1% (=1 g/l) 1.098, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.13
This classifies the protein as stable.
Aliphatic index: 103.69
Grand average of hydropathicity (GRAVY): 0.005