ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDVDDLIYAC RAADEELLDE IIEKCPQELS RRDENGNSGL HMASANGHIA VVQKIIPYLN 

        70         80         90        100        110        120 
KEVINAQNES GNTAMHWAAL NGHAEICKLL LEAGGDPHIK NIYEKSPIYE ADIRNQQKVM 

       130        140 
DLFLDFEIAK GSEENTGDEE KLEDGI 

References and documentation are available.

Number of amino acids: 146

Molecular weight: 16280.17

Theoretical pI: 4.47

Amino acid composition: 
Ala (A)  14	  9.6%
Arg (R)   4	  2.7%
Asn (N)  11	  7.5%
Asp (D)  12	  8.2%
Cys (C)   3	  2.1%
Gln (Q)   5	  3.4%
Glu (E)  18	 12.3%
Gly (G)  10	  6.8%
His (H)   5	  3.4%
Ile (I)  14	  9.6%
Leu (L)  13	  8.9%
Lys (K)   9	  6.2%
Met (M)   4	  2.7%
Phe (F)   2	  1.4%
Pro (P)   4	  2.7%
Ser (S)   6	  4.1%
Thr (T)   2	  1.4%
Trp (W)   1	  0.7%
Tyr (Y)   4	  2.7%
Val (V)   5	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 13

Atomic composition:

Carbon      C	       704
Hydrogen    H	      1114
Nitrogen    N	       194
Oxygen      O	       235
Sulfur      S	         7

Formula: C704H1114N194O235S7
Total number of atoms: 2254

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11585
Abs 0.1% (=1 g/l)   0.712, assuming all pairs of Cys residues form cystines


Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.704, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.08
This classifies the protein as unstable.



Aliphatic index: 91.64

Grand average of hydropathicity (GRAVY): -0.503