ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDVDLASVEV VFAQKLACGE PATRQRALRV LHDWIRDQSA KKHFDDADLM RLCKGLHYVM 

        70         80         90        100        110        120 
WMQDKMILQE ELADRIGGLI NIFTSEEEKV RFVACFLKSL SKEWPHIDRW RMDKFLMEVR 

       130        140        150        160        170        180 
RMVRACFTHL AELKWKKDIR DEYWKVFQET TISTDKSFNE ALKFHFASIL LDELDAAGGL 

       190        200        210        220        230        240 
TKKQVTACLK PYIELLGNKD ISEYLFTSLY EEIFKTILQQ KSDLITAVEE GEEMDQGGIE 

       250        260        270        280        290        300 
FSYKEIGALL FEVGKQEHLN AKRRKKIYDL VKKFDKSSRG QDPLHFETPV PKERLTRHDY 

       310        320        330        340        350        360 
EEAEKKAIVL ANSFKQERKS SRKVKSQIKK RAREAAEAAR QENGDDVPDD EIAEVKKGNG 

       370        380        390 
KKTAVPKVKK GRPLLKAKGV GKKRMVGGLK RKAGKKN 

References and documentation are available.

Number of amino acids: 397

Molecular weight: 45870.11

Theoretical pI: 9.32

Amino acid composition: 
Ala (A)  31	  7.8%
Arg (R)  26	  6.5%
Asn (N)   8	  2.0%
Asp (D)  27	  6.8%
Cys (C)   5	  1.3%
Gln (Q)  15	  3.8%
Glu (E)  37	  9.3%
Gly (G)  22	  5.5%
His (H)   9	  2.3%
Ile (I)  20	  5.0%
Leu (L)  37	  9.3%
Lys (K)  53	 13.4%
Met (M)  10	  2.5%
Phe (F)  18	  4.5%
Pro (P)   9	  2.3%
Ser (S)  18	  4.5%
Thr (T)  14	  3.5%
Trp (W)   6	  1.5%
Tyr (Y)   8	  2.0%
Val (V)  24	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      2047
Hydrogen    H	      3299
Nitrogen    N	       575
Oxygen      O	       589
Sulfur      S	        15

Formula: C2047H3299N575O589S15
Total number of atoms: 6525

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45170
Abs 0.1% (=1 g/l)   0.985, assuming all pairs of Cys residues form cystines


Ext. coefficient    44920
Abs 0.1% (=1 g/l)   0.979, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.52
This classifies the protein as unstable.



Aliphatic index: 81.34

Grand average of hydropathicity (GRAVY): -0.632