ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDVDTAFLNS TMDEPIYVKQ PPGFVNERNP DYVWELYGGM YGLKQAPLLW NEHINNTLKK 

        70         80         90        100        110        120 
IGFCRHEGEH GLYFRSTSDG PIYIAVYVDD LLVAAPSPKI YDRVKQELTK LYSMKDLGKV 

       130        140        150        160        170        180 
DKFLGLNIHQ SSNGDITLSL QDYIAKAASE SEINTFKLTQ TPLCNSKPLF ETTSPHLKDI 

       190        200        210        220        230        240 
TPYQSIVGQL LFCANTGRPD ISYPVSLLSR FLREPRAIHL ESARRVLRYL YTTRSMCLKY 

       250        260        270        280        290        300 
RSGSQLALTV YCDASHGAIH DLPHSTGGYV TLLAGAPVTW SSKKLKGVIP VPSTEAEYIT 


ASETVMEI 

References and documentation are available.

Number of amino acids: 308

Molecular weight: 34507.49

Theoretical pI: 6.75

Amino acid composition: 
Ala (A)  18	  5.8%
Arg (R)  13	  4.2%
Asn (N)  11	  3.6%
Asp (D)  16	  5.2%
Cys (C)   5	  1.6%
Gln (Q)   9	  2.9%
Glu (E)  16	  5.2%
Gly (G)  19	  6.2%
His (H)   9	  2.9%
Ile (I)  18	  5.8%
Leu (L)  35	 11.4%
Lys (K)  18	  5.8%
Met (M)   6	  1.9%
Phe (F)   9	  2.9%
Pro (P)  19	  6.2%
Ser (S)  26	  8.4%
Thr (T)  22	  7.1%
Trp (W)   3	  1.0%
Tyr (Y)  18	  5.8%
Val (V)  18	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1555
Hydrogen    H	      2428
Nitrogen    N	       406
Oxygen      O	       459
Sulfur      S	        11

Formula: C1555H2428N406O459S11
Total number of atoms: 4859

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43570
Abs 0.1% (=1 g/l)   1.263, assuming all pairs of Cys residues form cystines


Ext. coefficient    43320
Abs 0.1% (=1 g/l)   1.255, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.26
This classifies the protein as stable.



Aliphatic index: 89.90

Grand average of hydropathicity (GRAVY): -0.223