ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDVGSCSVGN NPLAQLHKHT QQNKSLQFNQ KNNGRLNESP LQGTNKPGIS EAFISNVNAI 

        70         80         90        100        110        120 
SQENMANMQR FINGEPLIDD KRRMEIGPSS GRLPPFSNVH SLQTSANPTQ IKGVNDISHW 

       130        140        150        160        170        180 
SQEFQGSNSI QNRNADTGNS EKAWQRGSTT ASSRFQYPNT MMNNYAYASM NSLSGSRLQS 

       190        200        210        220        230        240 
PAFMNQQQSG RSKEGVNEQE QQPWTDQFEK LEKEVSENLD INDEIEKEEN VSEVEQNKPE 

       250        260        270        280        290        300 
TVEKEEGVYG DQYQSDFQEV WDSIHKDAEE VLPSELVNDD LNLGEDYLKY LGGRVNGNIE 

       310        320        330        340        350        360 
YAFQSNNEYF NNPNAYKIGC LLMENGAKLS EAALAFEAAV KEKPDHVDAW LRLGLVQTQN 

       370        380        390        400        410        420 
EKELNGISAL EECLKLDPKN LEAMKTLAIS YINEGYDMSA FTMLDKWAET KYPEIWSRIK 

       430        440        450        460        470        480 
QQDDKFQKEK GFTHIDMNAH ITKQFLQLAN NLSTIDPEIQ LCLGLLFYTK DDFDKTIDCF 

       490        500        510        520        530        540 
ESALRVNPND ELMWNRLGAS LANSNRSEEA IQAYHRALQL KPSFVRARYN LAVSSMNIGC 

       550        560        570        580        590        600 
FKEAAGYLLS VLSMHEVNTN NKKGDVGSLL NTYNDTVIET LKRVFIAMNR DDLLQEVKPG 

       610 
MDLKRFKGEF SF 

References and documentation are available.

Number of amino acids: 612

Molecular weight: 69324.16

Theoretical pI: 4.95

Amino acid composition: 
Ala (A)  39	  6.4%
Arg (R)  22	  3.6%
Asn (N)  60	  9.8%
Asp (D)  34	  5.6%
Cys (C)   6	  1.0%
Gln (Q)  39	  6.4%
Glu (E)  54	  8.8%
Gly (G)  35	  5.7%
His (H)  10	  1.6%
Ile (I)  28	  4.6%
Leu (L)  55	  9.0%
Lys (K)  40	  6.5%
Met (M)  18	  2.9%
Phe (F)  25	  4.1%
Pro (P)  21	  3.4%
Ser (S)  50	  8.2%
Thr (T)  23	  3.8%
Trp (W)   8	  1.3%
Tyr (Y)  18	  2.9%
Val (V)  27	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      3023
Hydrogen    H	      4709
Nitrogen    N	       845
Oxygen      O	       979
Sulfur      S	        24

Formula: C3023H4709N845O979S24
Total number of atoms: 9580

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    71195
Abs 0.1% (=1 g/l)   1.027, assuming all pairs of Cys residues form cystines


Ext. coefficient    70820
Abs 0.1% (=1 g/l)   1.022, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.36
This classifies the protein as unstable.



Aliphatic index: 72.06

Grand average of hydropathicity (GRAVY): -0.716