ProtParam
User-provided sequence:
10 20 30 40 50 60
MDVLKEVLSL DQDKFDQLKE TSRDKTNETD DPFENYLKDC KFKAPSNKDQ SPFAKLKSLQ
70 80 90 100 110 120
ETHSNNEAAI NIIIPQLIDY LTEFTNRLSN YTQDLDFIKK KSNELQSLLE YNSTKLAHIS
130 140 150 160 170 180
PMVNDLMIPP ELIDDIIKGK INESWQDNIT FIADKEEIYN KYRSNNLDQD NKDAENSAML
190 200 210 220 230 240
APKDFDKLCQ LLDILKNVIL ERSKRLIISK IKTLRSHNPV PSQRIQNKLL KVQKIFPFIR
250 260 270 280 290 300
DNNLSLALEL RQAYCYTMKW YYREYFSRYI RSLTILQFQQ IDSQFALGNG LSTTSVSGFN
310 320 330 340 350 360
NSPSLFFSNY LTTSASNAFY NKLPVTDEKI DKYFQIKKRL NILTQEDNTV MVSQIAENNT
370 380 390 400 410 420
TKNYIEIGFK NLNLAILDNC TVEYHFLKDF FAMNGDNFEE INGLLEQIFQ PTFDEATTYT
430 440 450 460 470 480
QQLIQYNYDI FGVLISIRVA NQLQFESERR GIPSMFDSFL NGQLIQLWPR FQQLVDFQCE
490 500 510 520 530 540
SLRKAAITTN VAKYAGNSST SNSSPLTSPH ELTVQFGKFL SSFLTLAITH KQSIDERSEP
550 560 570 580 590 600
LYNSIIRLRN DFETVMTKCS KKTKSPERFL ATNYMYLYNN LQQLHLHLNI NDSDAQNYNF
610 620 630 640
DSAENVGTKV ANDDDNDSSV PLIIRETENH FKTLVEAFTR N
References and
documentation are available.
Number of amino acids: 641
Molecular weight: 74332.93
Theoretical pI: 5.53
Total number of negatively charged residues (Asp + Glu): 82
Total number of positively charged residues (Arg + Lys): 71
Atomic composition:
Carbon C 3322
Hydrogen H 5183
Nitrogen N 879
Oxygen O 1024
Sulfur S 16
Formula: C
3322H
5183N
879O
1024S
16
Total number of atoms: 10424
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 55615
Abs 0.1% (=1 g/l) 0.748, assuming all pairs of Cys residues form cystines
Ext. coefficient 55240
Abs 0.1% (=1 g/l) 0.743, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.02
This classifies the protein as unstable.
Aliphatic index: 86.43
Grand average of hydropathicity (GRAVY): -0.509