ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDVLKLGYEL DQLSDLVEER TRLVSVLKLA PTSNDNVTLK RQLGSILELL QKCAPNDELI 

        70         80         90        100        110        120 
SRYNTILDKI PDTAVDKELY RFQQQVARNT DEVSKESLKK VRFKNDDELT VMYKDDDEQD 

       130        140        150        160        170        180 
EESPLPSTHT PYKDEPLQSQ LQSQSQPQPP QPMVSNQELF INQQQQLLEQ DSHLGALSQS 

       190        200        210        220        230        240 
IGRTHDISLD LNNEIVSQND SLLVDLENLI DNNGRNLNRA SRSMHGFNNS RFKDNGNCVI 

       250 
ILVLIVVLLL LLLVL 

References and documentation are available.

Number of amino acids: 255

Molecular weight: 29048.74

Theoretical pI: 4.65

Amino acid composition: 
Ala (A)   6	  2.4%
Arg (R)  12	  4.7%
Asn (N)  20	  7.8%
Asp (D)  23	  9.0%
Cys (C)   2	  0.8%
Gln (Q)  22	  8.6%
Glu (E)  17	  6.7%
Gly (G)   7	  2.7%
His (H)   4	  1.6%
Ile (I)  12	  4.7%
Leu (L)  42	 16.5%
Lys (K)  13	  5.1%
Met (M)   4	  1.6%
Phe (F)   5	  2.0%
Pro (P)  11	  4.3%
Ser (S)  22	  8.6%
Thr (T)  10	  3.9%
Trp (W)   0	  0.0%
Tyr (Y)   5	  2.0%
Val (V)  18	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1264
Hydrogen    H	      2060
Nitrogen    N	       354
Oxygen      O	       415
Sulfur      S	         6

Formula: C1264H2060N354O415S6
Total number of atoms: 4099

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7575
Abs 0.1% (=1 g/l)   0.261, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.256, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.74
This classifies the protein as unstable.



Aliphatic index: 105.41

Grand average of hydropathicity (GRAVY): -0.507