ProtParam
User-provided sequence:
10 20 30 40 50 60
MDVLNKSIQT ALEALPKTSS SSSDGVSLVS LKCQLLLSYV QKLAFLMLVK LDDESFLQHQ
70 80 90 100 110 120
DVVEKLVQLR IEIEKIRPLE NRIQYSVDKL LRAAGRKEEI GSIKEPENNG NDKDSQDSLK
130 140 150 160 170 180
LHYKPNLSEF ADDSDGPASE NNVVKEDDKS SISSEDEEEE LRSAKDGIYR PPRIRAVTMD
190 200 210 220 230 240
SEKRTRHRPN HLVDEFVSSD MSSVPQSMPS VGSNLEKRGR VIHADERELQ KMRERIEYEE
250 260 270 280 290 300
SNYTRLPKLS KKDLKKSKRV KKHDYGGEDW SLLDRKFHDD DFSNRKLDLT SRAKRRANFE
310 320
DVNDGSLASP VQIGQSYRKR KKNLKRR
References and
documentation are available.
Number of amino acids: 327
Molecular weight: 37645.33
Theoretical pI: 8.63
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 1624
Hydrogen H 2657
Nitrogen N 487
Oxygen O 526
Sulfur S 7
Formula: C
1624H
2657N
487O
526S
7
Total number of atoms: 5301
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.463, assuming all pairs of Cys residues form cystines
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.463, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.09
This classifies the protein as unstable.
Aliphatic index: 77.77
Grand average of hydropathicity (GRAVY): -0.991