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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDVLNKSIQT ALEALPKTSS SSSDGVSLVS LKCQLLLSYV QKLAFLMLVK LDDESFLQHQ

70 80 90 100 110 120
DVVEKLVQLR IEIEKIRPLE NRIQYSVDKL LRAAGRKEEI GSIKEPENNG NDKDSQDSLK

130 140 150 160 170 180
LHYKPNLSEF ADDSDGPASE NNVVKEDDKS SISSEDEEEE LRSAKDGIYR PPRIRAVTMD

190 200 210 220 230 240
SEKRTRHRPN HLVDEFVSSD MSSVPQSMPS VGSNLEKRGR VIHADERELQ KMRERIEYEE

250 260 270 280 290 300
SNYTRLPKLS KKDLKKSKRV KKHDYGGEDW SLLDRKFHDD DFSNRKLDLT SRAKRRANFE

310 320
DVNDGSLASP VQIGQSYRKR KKNLKRR


References and documentation are available.

Number of amino acids: 327 Molecular weight: 37645.33 Theoretical pI: 8.63
Amino acid composition: 
Ala (A) 13 4.0% Arg (R) 28 8.6% Asn (N) 15 4.6% Asp (D) 30 9.2% Cys (C) 1 0.3% Gln (Q) 12 3.7% Glu (E) 29 8.9% Gly (G) 12 3.7% His (H) 7 2.1% Ile (I) 13 4.0% Leu (L) 34 10.4% Lys (K) 34 10.4% Met (M) 6 1.8% Phe (F) 7 2.1% Pro (P) 12 3.7% Ser (S) 39 11.9% Thr (T) 6 1.8% Trp (W) 1 0.3% Tyr (Y) 8 2.4% Val (V) 20 6.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 59 Total number of positively charged residues (Arg + Lys): 62 Atomic composition: Carbon C 1624 Hydrogen H 2657 Nitrogen N 487 Oxygen O 526 Sulfur S 7 Formula: C1624H2657N487O526S7 Total number of atoms: 5301 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 17420 Abs 0.1% (=1 g/l) 0.463, assuming all pairs of Cys residues form cystines Ext. coefficient 17420 Abs 0.1% (=1 g/l) 0.463, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 57.09 This classifies the protein as unstable. Aliphatic index: 77.77 Grand average of hydropathicity (GRAVY): -0.991