ProtParam
User-provided sequence:
10 20 30 40 50 60
MDVPRPAFKC FDDDGRLKRS GTVWTASAHI ITAVIGSGVL SLAWAIGQLG WIAGPTVMLL
70 80 90 100 110 120
FSFVTYYSST LLSDCYRTGD PVSGKRNYTY MDAVRSILGG FRFKICGLIQ YLNLFGITVG
130 140 150 160 170 180
YTIAASISMM AIKRSNCFHE SGGKNPCHMS SNPYMIMFGV TEILLSQIKD FDQIWWLSIV
190 200 210 220 230 240
AAIMSFTYSA IGLALGIIQV AANGVVKGSL TGISIGAVTQ TQKIWRTFQA LGDIAFAYSY
250 260 270 280 290 300
SVVLIEIQDT VRSPPAESKT MKIATRISIA VTTTFYMLCG CMGYAAFGDK APGNLLTGFG
310 320 330 340 350 360
FYNPFWLLDV ANAAIVIHLV GAYQVFAQPI FAFIEKQAAA RFPDSDLVTK EYEIRIPGFR
370 380 390 400 410 420
SPYKVNVFRA VYRSGFVVLT TVISMLMPFF NDVVGILGAL GFWPLTVYFP VEMYIRQRKV
430 440 450 460
ERWSMKWVCL QMLSCGCLMI TLVAGVGSIA GVMLDLKVYK PFKTTY
References and
documentation are available.
Number of amino acids: 466
Molecular weight: 51428.53
Theoretical pI: 9.21
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 2374
Hydrogen H 3668
Nitrogen N 586
Oxygen O 630
Sulfur S 29
Formula: C
2374H
3668N
586O
630S
29
Total number of atoms: 7287
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 88405
Abs 0.1% (=1 g/l) 1.719, assuming all pairs of Cys residues form cystines
Ext. coefficient 87780
Abs 0.1% (=1 g/l) 1.707, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.80
This classifies the protein as stable.
Aliphatic index: 99.59
Grand average of hydropathicity (GRAVY): 0.491