ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDVRAALQCF FSALSGRFTG KKLGLEIYSI QYKMSNSGGS SPFLESPGGS PDVGSTNGQS 

        70         80         90        100        110        120 
NRQIQALQFK LNTLQNEYEI EKLQLQKQTN ILEKKYKATI DELEKALNDT KYLYESNDKL 

       130        140        150        160        170        180 
EQELKSLKER SANSMNDKDK CIEELRTTLQ NKDLEMETLR QQYDSKLSKV TNQCDHFKLE 

       190        200        210        220        230        240 
AESSHSLLMK YEKEIKRQSV DIKDLQHQVM EKDDELSSVK ASKMINSHPN YSTEEFNELT 

       250        260        270        280        290        300 
EMNKMIQDQV QYTKELELAN MQQANELKKL KQSQDTSTFW KLENEKLQNK LSQLHVLESQ 

       310        320        330        340        350        360 
YENLQLENID LKSKLTKWEI YNDSDDDDDN NVNNNDNNNN NKNDNNNDNN NDTSNNNNIN 

       370        380        390        400        410        420 
NNNRTKNNIR NNPEEIIRDW KLTKKECLIL TDMNDKLRLD NNNLKLLNDE MALERNQILD 

       430        440        450        460        470        480 
LNKNYENNIV NLKRLNHELE QQKSLSFEEC RLLREQLDGL YSAQNNALLE VENSETHASN 

       490        500        510        520        530        540 
KNVNEDMNNL IDTYKNKTED LTNELKKLND QLLSNSNDVE TQRKKRKLTS DQIGLNYSQR 

       550        560        570        580        590        600 
LNELQLENVS VSRELSKAQT TIQLLQEKLE KLTKLKEKKI RILQLRDGPF IKDQFIKKNK 

       610        620        630        640        650        660 
LLLLEKENAD LLNELKKNNP AVETVPISVY DSLNFELKQF EQEVFKSNKR FSRLKQVFNN 

       670        680        690        700        710        720 
KSLEFIDVVN SLLGFKLEFQ QDSRVKIFSC FKPEKYLIAD LNENTLKSNL DADIEGWDDL 

       730        740 
MNLWVEDRGQ LPCFLATITL RLWEQRQAK 

References and documentation are available.

Number of amino acids: 749

Molecular weight: 87651.31

Theoretical pI: 5.32

Amino acid composition: 
Ala (A)  22	  2.9%
Arg (R)  27	  3.6%
Asn (N)  94	 12.6%
Asp (D)  52	  6.9%
Cys (C)   7	  0.9%
Gln (Q)  52	  6.9%
Glu (E)  73	  9.7%
Gly (G)  15	  2.0%
His (H)   7	  0.9%
Ile (I)  33	  4.4%
Leu (L) 105	 14.0%
Lys (K)  81	 10.8%
Met (M)  14	  1.9%
Phe (F)  22	  2.9%
Pro (P)  10	  1.3%
Ser (S)  54	  7.2%
Thr (T)  33	  4.4%
Trp (W)   6	  0.8%
Tyr (Y)  18	  2.4%
Val (V)  24	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 125
Total number of positively charged residues (Arg + Lys): 108

Atomic composition:

Carbon      C	      3804
Hydrogen    H	      6139
Nitrogen    N	      1077
Oxygen      O	      1251
Sulfur      S	        21

Formula: C3804H6139N1077O1251S21
Total number of atoms: 12292

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60195
Abs 0.1% (=1 g/l)   0.687, assuming all pairs of Cys residues form cystines


Ext. coefficient    59820
Abs 0.1% (=1 g/l)   0.682, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.54
This classifies the protein as unstable.



Aliphatic index: 84.09

Grand average of hydropathicity (GRAVY): -0.977