ProtParam
User-provided sequence:
10 20 30 40 50 60
MDVRIKCNSE EPESPEQKIL ASSQRLLQFT NCRLVRDHRI IHDDLWVRDG RIVNPEPVFF
70 80 90 100 110 120
DERTKAHCRI DCGGAIIAPG YIDLQINGGY GVDFSYDTET IEEGVATVAR GLVKSGVTSF
130 140 150 160 170 180
CPTLVTSPSD SYHTILPRIP AEVPKGAGIL GIHAEGPFIN PQKKGAHPEH CIQTIDKGLS
190 200 210 220 230 240
TLKETYGSLE RIKIITLAPE KVTDPEVIGQ LVERGITVAL GHSMASLSDG ERAVQQGATL
250 260 270 280 290 300
ITHLFNAMLP FHHRDPGLVG LLASDAVPHG RTVYFGIISD GVHTHPAALR IAYRTHPQGL
310 320 330 340 350 360
ILVTDAISAL GLEEGVHHIG QLPLQVKQGK AFIAGTETLC GSIAPMDECV RIFQKATDCS
370 380 390 400 410
VVYAIEAATL HPAQCLKIEK QKGTLDFGSD ADFVLLDDQL RVLSTWIAGV CVHRTVK
References and
documentation are available.
Number of amino acids: 417
Molecular weight: 45282.93
Theoretical pI: 6.01
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 2011
Hydrogen H 3213
Nitrogen N 561
Oxygen O 597
Sulfur S 15
Formula: C
2011H
3213N
561O
597S
15
Total number of atoms: 6397
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23545
Abs 0.1% (=1 g/l) 0.520, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.506, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.83
This classifies the protein as stable.
Aliphatic index: 99.38
Grand average of hydropathicity (GRAVY): -0.016