ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDVRSLPSNG VATIQDSAPT AATILGSSAA TLGRHLSRRL VQAGVTDIFT VPGDFNLSLL 

        70         80         90        100        110        120 
DQLIANPELN NIGCCNELNA GYAADGYARS RGVGACVVTF TVGGLSVLNA IAGAYSENLP 

       130        140        150        160        170        180 
VICIVGGPNS NDFGTNRILH HTIGLPDFSQ ELRCFQTVTC YQAVVNHLED AHEQIDKAIA 

       190        200        210        220        230        240 
TALRESKPVY ISISCNLAAI PHPTFASYPV PFDLTPRLSN KDCLEAAVEA TLEFLNKAVK 

       250        260        270        280        290        300 
PVMVGGPKLR VAKARDAFVE LADASGYPVA VMPSAKGFVP ENHPHFIGTY WGAVSTLFCS 

       310        320        330        340        350        360 
EIVESADAYI FAGPIFNDYS SVGYSLLLKK EKAIIVHPDS VVVANGPTFG CVRMSEFFRE 

       370        380        390        400        410        420 
LAKRVKPNKT AYENYHRIFV PEGKPLKCKP REPLRINAMF QHIQKMLSNE TAVIAETGDS 

       430        440        450        460        470        480 
WFNCQKLKLP KGCGYEFQMQ YGSIGWSVGA TLGYAQATPE KRVLSFIGDG SFQVTAQDVS 

       490        500        510        520        530        540 
TMIRNGQKTI IFLINNGGYT IEVEIHDGPY NVIKNWNYTG LVDAIHNGEG KCWTTKVRYE 

       550        560        570        580        590 
EELVEAINTA TLEKKDSLCF IEVIVHKDDT SKELLEWGSR VSAANGRPPN PQ 

References and documentation are available.

Number of amino acids: 592

Molecular weight: 64481.55

Theoretical pI: 6.09

Amino acid composition: 
Ala (A)  55	  9.3%
Arg (R)  23	  3.9%
Asn (N)  34	  5.7%
Asp (D)  25	  4.2%
Cys (C)  15	  2.5%
Gln (Q)  17	  2.9%
Glu (E)  35	  5.9%
Gly (G)  46	  7.8%
His (H)  14	  2.4%
Ile (I)  38	  6.4%
Leu (L)  46	  7.8%
Lys (K)  30	  5.1%
Met (M)   8	  1.4%
Phe (F)  26	  4.4%
Pro (P)  33	  5.6%
Ser (S)  38	  6.4%
Thr (T)  34	  5.7%
Trp (W)   6	  1.0%
Tyr (Y)  20	  3.4%
Val (V)  49	  8.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2884
Hydrogen    H	      4504
Nitrogen    N	       776
Oxygen      O	       856
Sulfur      S	        23

Formula: C2884H4504N776O856S23
Total number of atoms: 9043

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63675
Abs 0.1% (=1 g/l)   0.987, assuming all pairs of Cys residues form cystines


Ext. coefficient    62800
Abs 0.1% (=1 g/l)   0.974, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.03
This classifies the protein as stable.



Aliphatic index: 88.63

Grand average of hydropathicity (GRAVY): -0.058