ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDYEEILFGL QPILNASSIK DVPMNDVYLG SYLAVMDQLA VSLREPSNRD IVGKTGLLLN 

        70         80         90        100        110        120 
LVRVLEQALD ICFHDTSISI NDKIAFYEIS SEVIRCIANA IIDNDDNREI LLDSGGKKLL 

       130        140        150        160        170        180 
NYYIGGVLQL DEISSDKSED SLVDKLQMRS VVLLRNFCIG NLKYTENLAP FIRGPLFVLL 

       190        200        210        220        230        240 
KTTQYSYLSS PEKVVLGSDL LNDILKVNYS NVQISDLFFL SQYIKKISSN VQNKELQAME 

       250        260        270        280        290        300 
DGAVEAYSNT ETQKFAGQGN QEYIEKEEED DEEDVNCELL LNLSTCLETI VAKDETINFT 

       310        320        330        340        350        360 
NEEQLVLSMQ KNLILSLVCL ESKTFNNKLI VMRRLISCAG NISANLTNSN KREQSLCIET 

       370        380        390        400        410        420 
IKSSASSYAL AAALMILCNS VASKSDAVAL LKLISLSELI QVGSLLQDPL QYQGFLDLLR 

       430        440        450        460        470        480 
KLLNLENTMW LDIKDLFTLF QIMRRCHEQT KYYNNLRSLL TNLLNKTLTV LPSSKIHNSI 

       490        500        510        520        530        540 
SSDPTIISFI AEHGTLTSCI AMDKLLVSKK ALPKEAITSL WDSIFKFQNL GQAEQLSISD 

       550        560        570        580        590        600 
LFHITKTVGI YLKDSSVTAD VNPIENILFK DYIQKLTLIL ETILSFKENK DKGSESCFNN 

       610        620        630 
GKFIAGIILN IVKNTKCLTP EEQNLEALAK SFF 

References and documentation are available.

Number of amino acids: 633

Molecular weight: 70992.78

Theoretical pI: 4.92

Amino acid composition: 
Ala (A)  32	  5.1%
Arg (R)  15	  2.4%
Asn (N)  47	  7.4%
Asp (D)  37	  5.8%
Cys (C)  13	  2.1%
Gln (Q)  27	  4.3%
Glu (E)  43	  6.8%
Gly (G)  23	  3.6%
His (H)   5	  0.8%
Ile (I)  56	  8.8%
Leu (L)  96	 15.2%
Lys (K)  45	  7.1%
Met (M)  11	  1.7%
Phe (F)  24	  3.8%
Pro (P)  12	  1.9%
Ser (S)  62	  9.8%
Thr (T)  31	  4.9%
Trp (W)   2	  0.3%
Tyr (Y)  19	  3.0%
Val (V)  33	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      3168
Hydrogen    H	      5121
Nitrogen    N	       809
Oxygen      O	       980
Sulfur      S	        24

Formula: C3168H5121N809O980S24
Total number of atoms: 10102

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40060
Abs 0.1% (=1 g/l)   0.564, assuming all pairs of Cys residues form cystines


Ext. coefficient    39310
Abs 0.1% (=1 g/l)   0.554, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.79
This classifies the protein as unstable.



Aliphatic index: 113.82

Grand average of hydropathicity (GRAVY): 0.015