ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDYFNIKQNY YTGNFVQCLQ EIEKFSKVTD NTLLFYKAKT LLALGQYQSQ DPTSKLGKVL 

        70         80         90        100        110        120 
DLYVQFLDTK NIEELENLLK DKQNSPYELY LLATAQAILG DLDKSLETCV EGIDNDEAEG 

       130        140        150        160        170        180 
TTELLLLAIE VALLNNNVST ASTIFDNYTN AIEDTVSGDN EMILNLAESY IKFATNKETA 

       190        200        210        220        230        240 
TSNFYYYEEL SQTFPTWKTQ LGLLNLHLQQ RNIAEAQGIV ELLLSDYYSV EQKENAVLYK 

       250        260        270        280        290 
PTFLANQITL ALMQGLDTED LTNQLVKLDH EHAFIKHHQE IDAKFDELVR KYDTSN 

References and documentation are available.

Number of amino acids: 296

Molecular weight: 33828.98

Theoretical pI: 4.44

Amino acid composition: 
Ala (A)  20	  6.8%
Arg (R)   2	  0.7%
Asn (N)  23	  7.8%
Asp (D)  20	  6.8%
Cys (C)   2	  0.7%
Gln (Q)  19	  6.4%
Glu (E)  26	  8.8%
Gly (G)  10	  3.4%
His (H)   5	  1.7%
Ile (I)  15	  5.1%
Leu (L)  45	 15.2%
Lys (K)  20	  6.8%
Met (M)   3	  1.0%
Phe (F)  12	  4.1%
Pro (P)   4	  1.4%
Ser (S)  14	  4.7%
Thr (T)  25	  8.4%
Trp (W)   1	  0.3%
Tyr (Y)  17	  5.7%
Val (V)  13	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1519
Hydrogen    H	      2361
Nitrogen    N	       375
Oxygen      O	       487
Sulfur      S	         5

Formula: C1519H2361N375O487S5
Total number of atoms: 4747

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30955
Abs 0.1% (=1 g/l)   0.915, assuming all pairs of Cys residues form cystines


Ext. coefficient    30830
Abs 0.1% (=1 g/l)   0.911, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.34
This classifies the protein as stable.



Aliphatic index: 98.55

Grand average of hydropathicity (GRAVY): -0.337