ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDYKEEQKQE LEVLESIYPD ELRIINDEYP KIKFEVAIKL ELDTGDSTSV LTKEHTIIAE 

        70         80         90        100        110        120 
FKLPENYPDE PCLISLEAQE VALNDNEEDN EEDEDEVEYD DHGNKVLKKF ENLPDLISFK 

       130        140        150        160        170        180 
GYLPELTVQL ESQIETDMLL GMQMCFALIS SIKERCEQWY SEQLNKLEKQ YELEAQEREK 

       190        200        210        220        230        240 
KEQAKFHGTK VTRESYLEWR SKFRQELKLD ERDQVRRMKA HHGKLTGKQM FEQGVVGTGD 

       250        260 
EYMEEDDASV DDVAKGLAKT EIANQ 

References and documentation are available.

Number of amino acids: 265

Molecular weight: 31030.62

Theoretical pI: 4.50

Amino acid composition: 
Ala (A)  12	  4.5%
Arg (R)   9	  3.4%
Asn (N)   9	  3.4%
Asp (D)  21	  7.9%
Cys (C)   3	  1.1%
Gln (Q)  16	  6.0%
Glu (E)  44	 16.6%
Gly (G)  11	  4.2%
His (H)   5	  1.9%
Ile (I)  13	  4.9%
Leu (L)  27	 10.2%
Lys (K)  25	  9.4%
Met (M)   7	  2.6%
Phe (F)   8	  3.0%
Pro (P)   7	  2.6%
Ser (S)  12	  4.5%
Thr (T)  11	  4.2%
Trp (W)   2	  0.8%
Tyr (Y)  10	  3.8%
Val (V)  13	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1360
Hydrogen    H	      2136
Nitrogen    N	       354
Oxygen      O	       454
Sulfur      S	        10

Formula: C1360H2136N354O454S10
Total number of atoms: 4314

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26025
Abs 0.1% (=1 g/l)   0.839, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.835, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.38
This classifies the protein as stable.



Aliphatic index: 77.62

Grand average of hydropathicity (GRAVY): -0.891