ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDYMKLGLKS RKTGIDVKQD IPKDEYSMEN IDDFFKDDET SLISMRRKSR RKSSLFLPST 

        70         80         90        100        110        120 
LNGDTKNVLP PFLQSYKSQD DEVVQSPSGK GDGSRRSSLL SHQSNFLSPA NDFEPIEEEP 

       130        140        150        160        170        180 
EQEENDIRGN DFATPITQKL SKPTYKRKYS TRYSLDTSES PSVRLTPDRI TNKNVYSDVP 

       190        200        210        220        230        240 
DLVADEDDDD RVNTSLNTSD NALLEDELED DGFIPESEED GDYIESDSSL DSGSDSASDS 

       250        260        270        280        290        300 
DGDNTYQEVE EEAEVNTNDN EDDYIRRQAS DVVRTDSIID RNGLRKSTRV KVAPLQYWRN 

       310        320        330        340        350        360 
EKIVYKRKSN KPVLDIDKIV TYDESEDEEE ILAAQRRKKQ KKKPTPTRPY NYVPTGRPRG 

       370        380        390        400        410        420 
RPKKDPNAKE NLIPEDPNED IIERIESGGI ENGEWLKHGI LEANVKISDT KEETKDEIIA 

       430        440        450        460        470        480 
FAPNLSQTEQ VKDTKDENFA LEIMFDKHKE YFASGILKLP AISGQKKLSN SFRTYITFHV 

       490        500        510        520        530        540 
IQGIVEVTVC KNKFLSVKGS TFQIPAFNEY AIANRGNDEA KMFFVQVTVS EDANDDNDKE 


LDSTFDTFG 

References and documentation are available.

Number of amino acids: 549

Molecular weight: 62472.64

Theoretical pI: 4.65

Amino acid composition: 
Ala (A)  22	  4.0%
Arg (R)  30	  5.5%
Asn (N)  34	  6.2%
Asp (D)  61	 11.1%
Cys (C)   1	  0.2%
Gln (Q)  18	  3.3%
Glu (E)  52	  9.5%
Gly (G)  24	  4.4%
His (H)   4	  0.7%
Ile (I)  34	  6.2%
Leu (L)  34	  6.2%
Lys (K)  47	  8.6%
Met (M)   6	  1.1%
Phe (F)  21	  3.8%
Pro (P)  28	  5.1%
Ser (S)  52	  9.5%
Thr (T)  32	  5.8%
Trp (W)   2	  0.4%
Tyr (Y)  18	  3.3%
Val (V)  29	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 113
Total number of positively charged residues (Arg + Lys): 77

Atomic composition:

Carbon      C	      2713
Hydrogen    H	      4272
Nitrogen    N	       748
Oxygen      O	       930
Sulfur      S	         7

Formula: C2713H4272N748O930S7
Total number of atoms: 8670

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37820
Abs 0.1% (=1 g/l)   0.605, assuming all pairs of Cys residues form cystines


Ext. coefficient    37820
Abs 0.1% (=1 g/l)   0.605, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.47
This classifies the protein as unstable.



Aliphatic index: 67.63

Grand average of hydropathicity (GRAVY): -0.976