ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDYPDPDTIR ILITTDNHVG YNENDPITGD DSWKTFHEVM MLAKNNNVDM VVQSGDLFHV 

        70         80         90        100        110        120 
NKPSKKSLYQ VLKTLRLCCM GDKPCELELL SDPSQVFHYD EFTNVNYEDP NFNISIPVFG 

       130        140        150        160        170        180 
ISGNHDDASG DSLLCPMDIL HATGLINHFG KVIESDKIKV VPLLFQKGST KLALYGLAAV 

       190        200        210        220        230        240 
RDERLFRTFK DGGVTFEVPT MREGEWFNLM CVHQNHTGHT NTAFLPEQFL PDFLDMVIWG 

       250        260        270        280        290        300 
HEHECIPNLV HNPIKNFDVL QPGSSVATSL CEAEAQPKYV FILDIKYGEA PKMTPIPLET 

       310        320        330        340        350        360 
IRTFKMKSIS LQDVPHLRPH DKDATSKYLI EQVEEMIRDA NEETKQKLAD DGEGDMVAEL 

       370        380        390        400        410        420 
PKPLIRLRVD YSAPSNTQSP IDYQVENPRR FSNRFVGRVA NGNNVVQFYK KRSPVTRSKK 

       430        440        450        460        470        480 
SGINGTSISD RDVEKLFSES GGELEVQTLV NDLLNKMQLS LLPEVGLNEA VKKFVDKDEK 

       490        500        510        520        530        540 
TALKEFISHE ISNEVGILST NEEFLRTDDA EEMKALIKQV KRANSVRPTP PKENDETNFA 

       550        560        570        580        590        600 
FNGNGLDSFR SSNREVRTGS PDITQSHVDN ESRITHISQA ESSKPTSKPK RVRTATKKKI 

       610        620        630        640        650        660 
PAFSDSTVIS DAENELGDNN DAQDDVDIDE NDIIMVSTDE EDASYGLLNG RKTKTKTRPA 

       670        680        690 
ASTKTASRRG KGRASRTPKT DILGSLLAKK RK 

References and documentation are available.

Number of amino acids: 692

Molecular weight: 77650.48

Theoretical pI: 5.57

Amino acid composition: 
Ala (A)  34	  4.9%
Arg (R)  34	  4.9%
Asn (N)  44	  6.4%
Asp (D)  56	  8.1%
Cys (C)   7	  1.0%
Gln (Q)  20	  2.9%
Glu (E)  50	  7.2%
Gly (G)  37	  5.3%
His (H)  18	  2.6%
Ile (I)  38	  5.5%
Leu (L)  56	  8.1%
Lys (K)  54	  7.8%
Met (M)  16	  2.3%
Phe (F)  29	  4.2%
Pro (P)  38	  5.5%
Ser (S)  53	  7.7%
Thr (T)  45	  6.5%
Trp (W)   3	  0.4%
Tyr (Y)  13	  1.9%
Val (V)  47	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 106
Total number of positively charged residues (Arg + Lys): 88

Atomic composition:

Carbon      C	      3402
Hydrogen    H	      5409
Nitrogen    N	       951
Oxygen      O	      1080
Sulfur      S	        23

Formula: C3402H5409N951O1080S23
Total number of atoms: 10865

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36245
Abs 0.1% (=1 g/l)   0.467, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   0.462, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.65
This classifies the protein as stable.



Aliphatic index: 77.59

Grand average of hydropathicity (GRAVY): -0.598